Cell death occurs during the differentiation of 32D/FLT3/ITD and 32D/pBabe cells
| . | Day 0, % . | Day 1, % . | Day 11, % . |
|---|---|---|---|
| 32D/FLT3/ITD | 11.2 ± 2.5 | 11.1 ± 1.7 | 8.8 ± 0.6 |
| 32D/FLT3/ITD+G-CSF | 11.2 ± 2.5 | 8.6 ± 1.3 | 9.9 ± 0.4 |
| 32D/FLT3/ITD+G-CSF+CEP-701 | 11.2 ± 2.5 | 18.5 ± 1.1 | 36.5 ± 2.9 |
| 32D/pBabe+IL-3 | 6.6 ± 1.0 | 9.5 ± 0.3 | 11.8 ± 0.6 |
| 32D/pBabe+IL-3+CEP-701 | 6.6 ± 1.0 | 5.9 ± 0.3 | 6.1 ± 0.5 |
| 32D/pBabe+G-CSF | 6.6 ± 1.0 | 16.8 ± 1.6 | 42.7 ± 0.3 |
| . | Day 0, % . | Day 1, % . | Day 11, % . |
|---|---|---|---|
| 32D/FLT3/ITD | 11.2 ± 2.5 | 11.1 ± 1.7 | 8.8 ± 0.6 |
| 32D/FLT3/ITD+G-CSF | 11.2 ± 2.5 | 8.6 ± 1.3 | 9.9 ± 0.4 |
| 32D/FLT3/ITD+G-CSF+CEP-701 | 11.2 ± 2.5 | 18.5 ± 1.1 | 36.5 ± 2.9 |
| 32D/pBabe+IL-3 | 6.6 ± 1.0 | 9.5 ± 0.3 | 11.8 ± 0.6 |
| 32D/pBabe+IL-3+CEP-701 | 6.6 ± 1.0 | 5.9 ± 0.3 | 6.1 ± 0.5 |
| 32D/pBabe+G-CSF | 6.6 ± 1.0 | 16.8 ± 1.6 | 42.7 ± 0.3 |
32D/FLT3/ITD cells were cultured in medium with or without G-CSF (20 ng/mL) in the presence or absence of CEP-701 (5 nM). 32D/pBabe cells were cultured either in medium containing IL-3 (1 ng/mL) with and without CEP-701 (5 nM) or in medium containing G-CSF (20 ng/mL). Early and late apoptotic cells were detected by annexin V/7-AAD staining followed by flow cytometry on days 0, 1, and 11.
Values are the mean percentages, ± SD, of cells from triplicate experiments.