CEP-701 overcomes the FLT3/ITD-mediated block of differentiation in 32D cells
| Cells . | G-CSF . | G-CSF + CEP-701 . | ||
|---|---|---|---|---|
| Day 0 . | Day 11 . | Day 0 . | day 11 . | |
| Myeloblasts, % | 84.7 ± 3.8 | 85.7 ± 5.9 | 88.0 ± 3.0 | 14.7 ± 4.7 |
| Intermediates, % | 15.3 ± 3.8 | 14.3 ± 5.9 | 12.0 ± 3.0 | 66.7 ± 5.5 |
| Granulocytes, % | 0 | 0 | 0 | 18.7 ± 3.5 |
| Cells . | G-CSF . | G-CSF + CEP-701 . | ||
|---|---|---|---|---|
| Day 0 . | Day 11 . | Day 0 . | day 11 . | |
| Myeloblasts, % | 84.7 ± 3.8 | 85.7 ± 5.9 | 88.0 ± 3.0 | 14.7 ± 4.7 |
| Intermediates, % | 15.3 ± 3.8 | 14.3 ± 5.9 | 12.0 ± 3.0 | 66.7 ± 5.5 |
| Granulocytes, % | 0 | 0 | 0 | 18.7 ± 3.5 |
Differential counts of 32D/FLT3/ITD cell cultures treated with G-CSF (20 ng/mL) with and without CEP-701 (5 nM) were performed on days 0 and 11.
Values are the mean percentages, ± SD, of cells from 3 independent counts examined by light microscopy after Wright-Giemsa staining of the cytospins. “Intermediates” include promyelocytes, myelocytes, and metamyelocytes.