FL stimulation slows, but does not block, differentiation of 32D/FLT3 cells
| Cells . | G-CSF . | G-CSF+FL . | ||||
|---|---|---|---|---|---|---|
| Day 0 . | Day 9 . | Day 12 . | Day 0 . | Day 9 . | Day 12 . | |
| Myeloblasts, % | 82.7 ± 5.0 | 13.7 ± 2.5 | 14.2 ± 1.2 | 83.7 ± 3.2 | 51.7 ± 2.5 | 17.5 ± 0.5 |
| Intermediates, % | 17.3 ± 5.0 | 63.7 ± 4.6 | 56.8 ± 2.8 | 16.3 ± 3.2 | 46.3 ± 3.8 | 59.7 ± 6.2 |
| Granulocytes, % | 0 | 22.6 ± 2.5 | 29.0 ± 2.8 | 0 | 2.0 ± 1.0 | 22.8 ± 6.6 |
| Cells . | G-CSF . | G-CSF+FL . | ||||
|---|---|---|---|---|---|---|
| Day 0 . | Day 9 . | Day 12 . | Day 0 . | Day 9 . | Day 12 . | |
| Myeloblasts, % | 82.7 ± 5.0 | 13.7 ± 2.5 | 14.2 ± 1.2 | 83.7 ± 3.2 | 51.7 ± 2.5 | 17.5 ± 0.5 |
| Intermediates, % | 17.3 ± 5.0 | 63.7 ± 4.6 | 56.8 ± 2.8 | 16.3 ± 3.2 | 46.3 ± 3.8 | 59.7 ± 6.2 |
| Granulocytes, % | 0 | 22.6 ± 2.5 | 29.0 ± 2.8 | 0 | 2.0 ± 1.0 | 22.8 ± 6.6 |
Differential counts of 32D/FLT3 cell cultures induced by G-CSF (20 ng/mL) with and without FL (100 ng/mL) were performed on days 0, 9, and 12.
Values are the mean percentages, ± SD, of cells from 3 independent counts examined by light microscopy after Wright-Giemsa staining of the cytospins. “Intermediates” include promyelocytes, myelocytes, and metamyelocytes.