Differentiation of 32Dcl3 cells is blocked by expression of FLT3/ITD
| Cells . | Day 0 . | Day 9 . | ||||
|---|---|---|---|---|---|---|
| 32D/pBabe . | 32D/FLT3 . | 32D/FLT3/ITD . | 32D/pBabe . | 32D/FLT3 . | 32DFLT3/ITD . | |
| Myeloblasts, % | 81.3 ± 2.5 | 85.7 ± 3.8 | 82.3 ± 4.5 | 14.3 ± 2.5 | 11.3 ± 3.2 | 85.3 ± 5.0 |
| Intermediates, % | 18.7 ± 2.5 | 14.3 ± 3.8 | 17.7 ± 4.5 | 64 ± 3.6 | 66.7 ± 4.5 | 14.7 ± 5.0 |
| Granulocytes, % | 0 | 0 | 0 | 21.7 ± 4.0 | 14.7 ± 5.0 | 0 |
| Cells . | Day 0 . | Day 9 . | ||||
|---|---|---|---|---|---|---|
| 32D/pBabe . | 32D/FLT3 . | 32D/FLT3/ITD . | 32D/pBabe . | 32D/FLT3 . | 32DFLT3/ITD . | |
| Myeloblasts, % | 81.3 ± 2.5 | 85.7 ± 3.8 | 82.3 ± 4.5 | 14.3 ± 2.5 | 11.3 ± 3.2 | 85.3 ± 5.0 |
| Intermediates, % | 18.7 ± 2.5 | 14.3 ± 3.8 | 17.7 ± 4.5 | 64 ± 3.6 | 66.7 ± 4.5 | 14.7 ± 5.0 |
| Granulocytes, % | 0 | 0 | 0 | 21.7 ± 4.0 | 14.7 ± 5.0 | 0 |
Differential counts of 32D/pBabe, 32D/FLT3, and 32D/FLT3/ITD cell cultures induced by G-CSF (20 ng/mL) were performed on days 0 and 9.
Values are the mean percentages, ± SD, of cells from 3 independent counts examined by light microscopy after Wright-Giemsa staining of the cytospins. “Intermediates” include promyelocytes, myelocytes, and metamyelocytes.