In vivo repopulating activity of sorted KLS cells
| Experiment no. . | WT KLS . | Fraction engrafted . | % Donor . | −/− KLS . | Fraction engrafted . | % Donor . |
|---|---|---|---|---|---|---|
| 1 | 250 | 3/4 | 53 ± 18 | na | na | — |
| 500 | 2/2 | 68 | na | na | — | |
| 2 | 50 | 0/4 | 0 | 2500 | 0/3 | 0 |
| 250 | 1/2 | 19 | 5000 | 0/3 | 0 | |
| 3 | 300 | 3/3 | 73 ± 4 | 2000 | 0/5 | 0 |
| Experiment no. . | WT KLS . | Fraction engrafted . | % Donor . | −/− KLS . | Fraction engrafted . | % Donor . |
|---|---|---|---|---|---|---|
| 1 | 250 | 3/4 | 53 ± 18 | na | na | — |
| 500 | 2/2 | 68 | na | na | — | |
| 2 | 50 | 0/4 | 0 | 2500 | 0/3 | 0 |
| 250 | 1/2 | 19 | 5000 | 0/3 | 0 | |
| 3 | 300 | 3/3 | 73 ± 4 | 2000 | 0/5 | 0 |
BM cells were obtained from both hind limbs of STAT5ab−/− or littermate wild-type mice, lineage depleted, and sorted by FACS for the Sca-1+c-kit+lin− (KLS) cells. Cells were mixed with 2 × 105 HW80 competitor cells and transplanted into lethally irradiated recipient HW80 mice (experiments no. 1 and no. 2). HW80 competition experiments were analyzed by hemoglobin electrophoresis for Hbscontribution, followed by densitometry of gels. For experiment no. 3, Ly-5.1 recipient mice were used, and analysis was by FACS for Ly-5.2+ cells in the peripheral blood. Analyses were performed at times between 12 and 16 weeks.
na indicates not attempted.