Clinical and laboratory data and CD59 expression by peripheral blood granulocytes and bone marrow CD34+cells from 3 PNH patients and a healthy volunteer
| Case no. . | Age/sex . | Hb, g/dL . | Reticulocytes, % . | WBC, × 109/L . | Granulocyte count, × 109/L . | BM cellularity, × 109/L . | Granulocytes* . | CD34+cells† . | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Neg, %‡ . | Int, %‡ . | Pos, %‡ . | Neg, %‡ . | Int, %‡ . | Pos, %‡ . | |||||||
| 1 | 69/M | 9.6 | 2.6 | 4.4 | 2.64 | 50 | 82.1 | 11.8 | 6.1 | 92.8 | ND | 7.2 |
| 2 | 56/M | 11.9 | 2.3 | 6.9 | 6.14 | 160 | 73.3 | ND | 26.7 | 82.6 | ND | 17.4 |
| 3 | 51/M | 6.1 | 3.2 | 3.7 | 1.62 | 80 | 36.1 | ND | 63.9 | 75.1 | ND | 24.9 |
| HV | 45/M | 14.7 | 1.4 | 5.5 | 2.56 | 100 | ND | ND | 98.7 | ND | ND | 98.7 |
| Case no. . | Age/sex . | Hb, g/dL . | Reticulocytes, % . | WBC, × 109/L . | Granulocyte count, × 109/L . | BM cellularity, × 109/L . | Granulocytes* . | CD34+cells† . | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Neg, %‡ . | Int, %‡ . | Pos, %‡ . | Neg, %‡ . | Int, %‡ . | Pos, %‡ . | |||||||
| 1 | 69/M | 9.6 | 2.6 | 4.4 | 2.64 | 50 | 82.1 | 11.8 | 6.1 | 92.8 | ND | 7.2 |
| 2 | 56/M | 11.9 | 2.3 | 6.9 | 6.14 | 160 | 73.3 | ND | 26.7 | 82.6 | ND | 17.4 |
| 3 | 51/M | 6.1 | 3.2 | 3.7 | 1.62 | 80 | 36.1 | ND | 63.9 | 75.1 | ND | 24.9 |
| HV | 45/M | 14.7 | 1.4 | 5.5 | 2.56 | 100 | ND | ND | 98.7 | ND | ND | 98.7 |
Hb indicates concentration of hemoglobin; WBC, white blood cell count; BM, bone marrow; HV, healthy volunteer; and ND, not detectable.
The granulocytes were examined by flow cytometric single-color analysis using a monoclonal antibody to CD59 as described in “Patients, materials, and methods.” The results are from the profiles shown in Figure 3.
Bone marrow MNCs were stained with monoclonal antibodies to CD59, CD34, and CD45 as described in “Patients, materials, and methods,” following separation of CD34+ cells by flow cytometry as shown in Figure 1. The results are from the profiles shown in Figure 3.
Neg, Int, and Pos refer to negative, intermediate, and positive populations by single-color flow cytometric analysis of peripheral granulocytes and by 3-color analysis of bone marrow MNCs, respectively, as defined in “Patients, materials, and methods.”