The amino acid composition of the CDR3 region within CD8α+βhigh, CD8α+βlow, and CD8αα αβ T cells
| CD8+high . | CD8α+βlow . | CD8αα . | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| BV . | N-D-N . | BJ . | Frequency . | BV . | N-D-N . | BJ . | Frequency . | BV . | N-D-N . | BJ . | Frequency . |
| BV21 | |||||||||||
| CASS | SGTAV | SNQP (1S6) | 2/19 | CASS | PVSGRL | VNEQ (2S1) | 3/16 | CASS | PVSGRL | VNEQ (2S1) | 9/16 |
| CASS | LDPSQGH | TQVF (2S3) | 2/19 | CASS | LDPSQGH | TQVF (2S3) | 3/16 | CASS | TTLAGT | VNEQ (2S1) | 1/16 |
| CASS | FVSGS | STDT (2S3) | 1/19 | CASS | FVSGS | STDT (2S3) | 3/16 | CASS | FVSGS | STDT (2S3) | 2/16 |
| CASS | TMG | ETQV (2S5) | 2/19 | CASS | PMR | TDTQ (2S3) | 1/16 | CASS | LEGRV | QETQ (2S5) | 1/16 |
| CASS | TPRTGS | SGAN (2S6) | 2/19 | CASS | PLSC | EQFF (2S1) | 1/16 | CASS | LVQGPP | DTQV (2S3) | 1/16 |
| CASS | PSL | NTEA (1S1) | 1/19 | CASS | SPGGA | TQVF (2S3) | 1/16 | CASS | LGGGSF | VEQV (2S7) | 1/16 |
| CASS | LGP | NTEA (1S1) | 1/19 | CASS | FVSVS | STDT (2S3) | 1/16 | CAS | RGLAA | QETQ (2S5) | 1/16 |
| CASS | SGTGALL | EQFF (2S1) | 1/19 | CASS | LGGGLSP | KNIQ (2S4) | 1/16 | — | — | — | — |
| CASS | FPMGDRASGGD | TGEL (2S2) | 1/19 | CASS | SVCGRLS | NEQF (2S1) | 1/16 | — | — | — | — |
| CASS | INPSQGH | TQVF (2S3) | 1/19 | CASS | FVSGRLSNA | QVFG (2S3) | 1/16 | — | — | — | — |
| CASS | LAGGP | TDTQ (2S3) | 1/19 | — | — | — | — | — | — | — | — |
| CASS | PSGTE | ETQV (2S5) | 1/19 | — | — | — | — | — | — | — | — |
| CASS | LARD | VEQV (2S7) | 1/19 | — | — | — | — | — | — | — | — |
| CASS | LGP | SVEQ (2S7) | 1/19 | — | — | — | — | — | — | — | — |
| CASS | FGIGDS | SVEQ (2S7) | 1/19 | — | — | — | — | — | — | — | — |
| BV20 | |||||||||||
| CAW | SPVSWA | GNTI (1S3) | 2/15 | CAW | SPVSWA | GNTI (1S3) | 9/11 | CAW | SPVSWA | GNTI (1S3) | 10/14 |
| CA | CRGCGR | STDT (2S3) | 1/15 | CAWS | GI | VNEQ (2S1) | 2/11 | CAWS | GI | VNEQ (2S1) | 1/14 |
| CA | QALI | STDT (2S3) | 1/15 | — | — | — | — | CAW | GLAD | TDTQ (2S3) | 1/14 |
| CAW | SAGTGG | VEQV (2S7) | 1/15 | — | — | — | — | CAW | SIGTSGM | VEQV (2S1) | 1/14 |
| CAW | SPSDGGRSLH | NEQF (2S1) | 1/15 | — | — | — | — | CAWS | FLSETGLI | GELF (2S2) | 1/14 |
| CAW | SDAGVH | EQVF (2S7) | 1/15 | — | — | — | — | — | — | — | — |
| CAW | IRTSGAN | NEQF (2S1) | 1/15 | — | — | — | — | — | — | — | — |
| CAW | MR | DTQV (2S3) | 1/15 | — | — | — | — | — | — | — | — |
| CAWS | QGAGE | EQFF (2S1) | 1/15 | — | — | — | — | — | — | — | — |
| CAWS | VLS | TDTQ (2S3) | 1/15 | — | — | — | — | — | — | — | — |
| CAWS | VMAR | VEQV (2S7) | 1/15 | — | — | — | — | — | — | — | — |
| CAWS | VEGV | NEKL (1S4) | 1/15 | — | — | — | — | — | — | — | — |
| CAWS | VTGGQ | DTQV (2S3) | 1/15 | — | — | — | — | — | — | — | — |
| CAWS | DPGD | EQVF (2S7) | 1/15 | — | — | — | — | — | — | — | — |
| BV14 | — | — | — | — | — | — | — | — | |||
| ASSL | GQSR | ETQV (2S5) | 7/11 | ASSL | GQSR | ETQV (2S5) | 10/11 | ASSL | GQSR | ETQV (2S5) | 6/13 |
| ASS | RGQG | VEQV (2S7) | 1/11 | ASSL | FPTGR | EKLF (1S4) | 1/11 | ASSL | FPTGR | EKLF (1S4) | 4/13 |
| ASS | SVGGRS | EQFF (2S1) | 1/11 | — | — | — | — | ASSL | EGQT | SPLH (1S6) | 2/13 |
| ASS | IAGIRTLD | TDTQ (2S3) | 1/11 | — | — | — | — | ASS | FELAGGA | ETQV (2S5) | 1/13 |
| ASS | SSGGS | SVNE (2S1) | 1/11 | — | — | — | — | — | — | — | — |
| CD8+high . | CD8α+βlow . | CD8αα . | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| BV . | N-D-N . | BJ . | Frequency . | BV . | N-D-N . | BJ . | Frequency . | BV . | N-D-N . | BJ . | Frequency . |
| BV21 | |||||||||||
| CASS | SGTAV | SNQP (1S6) | 2/19 | CASS | PVSGRL | VNEQ (2S1) | 3/16 | CASS | PVSGRL | VNEQ (2S1) | 9/16 |
| CASS | LDPSQGH | TQVF (2S3) | 2/19 | CASS | LDPSQGH | TQVF (2S3) | 3/16 | CASS | TTLAGT | VNEQ (2S1) | 1/16 |
| CASS | FVSGS | STDT (2S3) | 1/19 | CASS | FVSGS | STDT (2S3) | 3/16 | CASS | FVSGS | STDT (2S3) | 2/16 |
| CASS | TMG | ETQV (2S5) | 2/19 | CASS | PMR | TDTQ (2S3) | 1/16 | CASS | LEGRV | QETQ (2S5) | 1/16 |
| CASS | TPRTGS | SGAN (2S6) | 2/19 | CASS | PLSC | EQFF (2S1) | 1/16 | CASS | LVQGPP | DTQV (2S3) | 1/16 |
| CASS | PSL | NTEA (1S1) | 1/19 | CASS | SPGGA | TQVF (2S3) | 1/16 | CASS | LGGGSF | VEQV (2S7) | 1/16 |
| CASS | LGP | NTEA (1S1) | 1/19 | CASS | FVSVS | STDT (2S3) | 1/16 | CAS | RGLAA | QETQ (2S5) | 1/16 |
| CASS | SGTGALL | EQFF (2S1) | 1/19 | CASS | LGGGLSP | KNIQ (2S4) | 1/16 | — | — | — | — |
| CASS | FPMGDRASGGD | TGEL (2S2) | 1/19 | CASS | SVCGRLS | NEQF (2S1) | 1/16 | — | — | — | — |
| CASS | INPSQGH | TQVF (2S3) | 1/19 | CASS | FVSGRLSNA | QVFG (2S3) | 1/16 | — | — | — | — |
| CASS | LAGGP | TDTQ (2S3) | 1/19 | — | — | — | — | — | — | — | — |
| CASS | PSGTE | ETQV (2S5) | 1/19 | — | — | — | — | — | — | — | — |
| CASS | LARD | VEQV (2S7) | 1/19 | — | — | — | — | — | — | — | — |
| CASS | LGP | SVEQ (2S7) | 1/19 | — | — | — | — | — | — | — | — |
| CASS | FGIGDS | SVEQ (2S7) | 1/19 | — | — | — | — | — | — | — | — |
| BV20 | |||||||||||
| CAW | SPVSWA | GNTI (1S3) | 2/15 | CAW | SPVSWA | GNTI (1S3) | 9/11 | CAW | SPVSWA | GNTI (1S3) | 10/14 |
| CA | CRGCGR | STDT (2S3) | 1/15 | CAWS | GI | VNEQ (2S1) | 2/11 | CAWS | GI | VNEQ (2S1) | 1/14 |
| CA | QALI | STDT (2S3) | 1/15 | — | — | — | — | CAW | GLAD | TDTQ (2S3) | 1/14 |
| CAW | SAGTGG | VEQV (2S7) | 1/15 | — | — | — | — | CAW | SIGTSGM | VEQV (2S1) | 1/14 |
| CAW | SPSDGGRSLH | NEQF (2S1) | 1/15 | — | — | — | — | CAWS | FLSETGLI | GELF (2S2) | 1/14 |
| CAW | SDAGVH | EQVF (2S7) | 1/15 | — | — | — | — | — | — | — | — |
| CAW | IRTSGAN | NEQF (2S1) | 1/15 | — | — | — | — | — | — | — | — |
| CAW | MR | DTQV (2S3) | 1/15 | — | — | — | — | — | — | — | — |
| CAWS | QGAGE | EQFF (2S1) | 1/15 | — | — | — | — | — | — | — | — |
| CAWS | VLS | TDTQ (2S3) | 1/15 | — | — | — | — | — | — | — | — |
| CAWS | VMAR | VEQV (2S7) | 1/15 | — | — | — | — | — | — | — | — |
| CAWS | VEGV | NEKL (1S4) | 1/15 | — | — | — | — | — | — | — | — |
| CAWS | VTGGQ | DTQV (2S3) | 1/15 | — | — | — | — | — | — | — | — |
| CAWS | DPGD | EQVF (2S7) | 1/15 | — | — | — | — | — | — | — | — |
| BV14 | — | — | — | — | — | — | — | — | |||
| ASSL | GQSR | ETQV (2S5) | 7/11 | ASSL | GQSR | ETQV (2S5) | 10/11 | ASSL | GQSR | ETQV (2S5) | 6/13 |
| ASS | RGQG | VEQV (2S7) | 1/11 | ASSL | FPTGR | EKLF (1S4) | 1/11 | ASSL | FPTGR | EKLF (1S4) | 4/13 |
| ASS | SVGGRS | EQFF (2S1) | 1/11 | — | — | — | — | ASSL | EGQT | SPLH (1S6) | 2/13 |
| ASS | IAGIRTLD | TDTQ (2S3) | 1/11 | — | — | — | — | ASS | FELAGGA | ETQV (2S5) | 1/13 |
| ASS | SSGGS | SVNE (2S1) | 1/11 | — | — | — | — | — | — | — | — |
BV-Cβ amplification of sorted CD8α+βhigh, CD8α+βlow, and CD8αα αβ T cells in a healthy donor was pursued. BV21, BV20, and BV14 were selected for the sequence analysis using the same PCR products. Eleven to 19 randomly chosen clones were sequenced per each PCR product.
— indicates no data.