Surface antigen immunostainings of Raw 264.7 cells. Analysis was performed on cells cultured for 72 hours in the presence of IFN-γ or IL-4/IL-10 cytokines. After Fc receptor blocking with 2.4G2 F(ab)2 fragments, cells were directly labeled with α-H-2D^d–FITC, α-I-A^d/I-E^d–FITC, α-CD11b–FITC, α-B7-1–FITC, or α-B7-2–FITC. Cells were analyzed by FACS, and the results are represented as the percentage increase in mean fluorescence intensity (MFI) relative to corresponding isotype-matched control antibodies.