Predictors of R406-mediated cytotoxicity of DLBCL cell lines
| . | R406-induced apoptosis . | P . | |
|---|---|---|---|
| Positive . | Negative . | ||
| Cell-surface Ig | 10/10 | 3/5 | .1, NS |
| Tonic pSYK352 | 10/10 | 2/5 | .02 |
| BCR-induced pSYK525/6 | 10/10 | 0/5 | <.001 |
| BCR-induced p-BLNK | 9/10 | 0/5 | .002 |
| R406-decreased pSYK525/6 and p-BLNK | 10/10 | N/A | N/A |
| Comprehensive cluster–BCR | 10/10 | 0/5 | <.001 |
| . | R406-induced apoptosis . | P . | |
|---|---|---|---|
| Positive . | Negative . | ||
| Cell-surface Ig | 10/10 | 3/5 | .1, NS |
| Tonic pSYK352 | 10/10 | 2/5 | .02 |
| BCR-induced pSYK525/6 | 10/10 | 0/5 | <.001 |
| BCR-induced p-BLNK | 9/10 | 0/5 | .002 |
| R406-decreased pSYK525/6 and p-BLNK | 10/10 | N/A | N/A |
| Comprehensive cluster–BCR | 10/10 | 0/5 | <.001 |
Parameters evaluated as possible predictors of R406-mediated cytotoxicity of DLBCL cell lines included cell-surface immunoglobulin (IgG or IgM) expression; tonic phosphorylation of SYK352; BCR crosslink-induced phosphorylation of SYK525/6; BCR crosslink-induced BLNK phosphorylation; R406 inhibition of BCR crosslink-induced phosphoSYK525/6 and phospho-BLNK; and BCR comprehensive cluster designation. The P value is calculated using the Fisher exact probability test. NS indicates not significant; and N/A, not applicable.