Adhesion molecules expressed on human lung micro-vascular endothelial cells
| Marker . | . | Unstimulated endothelial cell control . | IL-1α–activated endothelial cells . |
|---|---|---|---|
| CD51 | Positivity | 87 ± 9% | 90 ± 8% |
| MCF | 340 ± 28 | 389 ± 3 | |
| PSR | Positivity | 5 ± 0.5% | 26 ± 3% |
| MCF | 38 ± 3 | 175 ± 21 | |
| CD36 | Positivity | 33 ± 18% | 30 ± 16% |
| MCF | 116 ± 29 | 115 ± 43 | |
| ICAM-1 | Positivity | 38 ± 7% | 92 ± 3% |
| MCF | 268 ± 33 | 607 ± 9 | |
| VCAM-1 | Positivity | 7 ± 3% | 53 ± 16% |
| MCF | 45 ± 8 | 322 ± 97 | |
| *P-selectin | Positivity | Not detected | Not detected |
| MCF |
| Marker . | . | Unstimulated endothelial cell control . | IL-1α–activated endothelial cells . |
|---|---|---|---|
| CD51 | Positivity | 87 ± 9% | 90 ± 8% |
| MCF | 340 ± 28 | 389 ± 3 | |
| PSR | Positivity | 5 ± 0.5% | 26 ± 3% |
| MCF | 38 ± 3 | 175 ± 21 | |
| CD36 | Positivity | 33 ± 18% | 30 ± 16% |
| MCF | 116 ± 29 | 115 ± 43 | |
| ICAM-1 | Positivity | 38 ± 7% | 92 ± 3% |
| MCF | 268 ± 33 | 607 ± 9 | |
| VCAM-1 | Positivity | 7 ± 3% | 53 ± 16% |
| MCF | 45 ± 8 | 322 ± 97 | |
| *P-selectin | Positivity | Not detected | Not detected |
| MCF |
Besides being characterized to be positive for acetylated LDL receptor, Factor VIII-related antigen and CD31 by the manufacturer, the lung endothelial cells used in the study were identified by flow cytometric analyses to be 90 to 98% positive for constitutively expressing antigens including CD146, and CD144 (two endothelial-specific antigens). Following incubation of endothelial cells for 6 hours in the absence, or presence of IL-1α (10 ng/mL), cells were harvested, labeled with desired test antibody or an equivalent amount of an isotype-matched negative control, and analyzed by flow cytometry as detailed. Values presented are the means (± SD) from 4 experiments. MCF, mean cell fluorescence, is a measure of receptor density per cell. *While P-selectin expression was undetectable under the experimental conditions described, P-selectin-positivity was noted on endothelial cells activated with A23187 for 30-minutes (see the results section for details). Multiple monoclonal antibodies were employed to identify the expression of ICAM-1 (clones 84H10, and BBIG-I1), VCAM-1 (clones 1G11, 51-10C9, and BBIG-V1), and P-selectin (clones CLB-Thromb/6, and AK-6). Similar expression profiles of adhesion markers were noted on the endothelial surface with different monoclonal antibodies. Results shown for ICAM-1, and VCAM-1 were obtained with clones 84H10, and 51-10C9, respectively.