Values of PF4, UFH and PHRs associated with peak light absorbance (A280nm)
| PF4 conc, μg/mL . | UFH conc, U/mL . | Peak A280nm . | PHR . |
|---|---|---|---|
| 200 | 5-20 | 1.38-1.43 | 2.6:1 to 1:2 |
| 100 | 5 | 0.881 | 1.3:1 |
| 50 | 2 | 0.411 | 1.6:1 |
| 25 | 1 | 0.271 | 1.6:1 |
| 10 | 0.25 | 0.068 | 2.6:1 |
| PF4 conc, μg/mL . | UFH conc, U/mL . | Peak A280nm . | PHR . |
|---|---|---|---|
| 200 | 5-20 | 1.38-1.43 | 2.6:1 to 1:2 |
| 100 | 5 | 0.881 | 1.3:1 |
| 50 | 2 | 0.411 | 1.6:1 |
| 25 | 1 | 0.271 | 1.6:1 |
| 10 | 0.25 | 0.068 | 2.6:1 |
Peak light absorption (A280nm) at each dose of PF4 from Figure 1 with calculated PF4-heparin ratios (PHRs) for corresponding PF4 and heparin concentration is shown.