Difference of p53 transcript levels in erythroid cells between wild-type and udusq1–/– mutants by real-time RT-PCR
| . | Crossing point . | Cycle no. difference* . | Relative fold difference . | |
|---|---|---|---|---|
| Wt sibling . | udusq1–/– . | |||
| Test 1 | 38.74 | 35.93 | 2.81 | 7.012845771 |
| Test 2 | 39.15 | 36.01 | 3.14 | 8.815240927 |
| Average | 2.975 | 7.914043349 | ||
| . | Crossing point . | Cycle no. difference* . | Relative fold difference . | |
|---|---|---|---|---|
| Wt sibling . | udusq1–/– . | |||
| Test 1 | 38.74 | 35.93 | 2.81 | 7.012845771 |
| Test 2 | 39.15 | 36.01 | 3.14 | 8.815240927 |
| Average | 2.975 | 7.914043349 | ||
*Elongation factor 1 alpha (elf1α) was used as an internal reference to normalize the reaction.