Mononuclear cell chimerism of dogs receiving marrow transplants from 2 donors
| Recipient . | Nucleated marrow cells × 108/kg . | Duration of engraftment (wks) . | GVHD . | Status: Last Observation (week) . | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Donor 1 . | Donor 2 . | Donor 1 (% chimerism) . | Donor 2 (% chimerism) . | Recipient (% chimerism) . | |||||||
| G158 | 3.8 | 2.0 | >47 (5) | >47 (1) | (94) | No | Trichimeric | E1 | (54) | ||
| G193 | 3.7 | 4.8 | >52 (61) | 16 (0) | [R] | (39) | No | Bichimeric | E1 | (52) | |
| G362 | 3.6 | 6.1 | >47 (28) | >47 (55) | (17) | No | Trichimeric | E1 | (47) | ||
| G513 | 4.8 | 2.7 | >56 (39) | >56 (34) | (27) | Yes | Trichimeric | E1 | (56) | ||
| G551 | 7.0 | 7.2 | >17 (35) | >17 (20) | (45) | No | Trichimeric | E1 | (37) | ||
| G631* | 4.9 | 4.3 | 10 (4.4) | [R] | 10 (4.0) | [R] | (92) | No | Host | E2 | (10) |
| G643 | 4.6 | 3.8 | >36 (33) | >36 (46) | (21) | No | Trichimeric | Alive | (36) | ||
| G664 | 2.8 | 2.0 | >28 (52) | 26 (0) | [R] | (48) | No | Bichimeric | Adopted | (28) | |
| Recipient . | Nucleated marrow cells × 108/kg . | Duration of engraftment (wks) . | GVHD . | Status: Last Observation (week) . | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Donor 1 . | Donor 2 . | Donor 1 (% chimerism) . | Donor 2 (% chimerism) . | Recipient (% chimerism) . | |||||||
| G158 | 3.8 | 2.0 | >47 (5) | >47 (1) | (94) | No | Trichimeric | E1 | (54) | ||
| G193 | 3.7 | 4.8 | >52 (61) | 16 (0) | [R] | (39) | No | Bichimeric | E1 | (52) | |
| G362 | 3.6 | 6.1 | >47 (28) | >47 (55) | (17) | No | Trichimeric | E1 | (47) | ||
| G513 | 4.8 | 2.7 | >56 (39) | >56 (34) | (27) | Yes | Trichimeric | E1 | (56) | ||
| G551 | 7.0 | 7.2 | >17 (35) | >17 (20) | (45) | No | Trichimeric | E1 | (37) | ||
| G631* | 4.9 | 4.3 | 10 (4.4) | [R] | 10 (4.0) | [R] | (92) | No | Host | E2 | (10) |
| G643 | 4.6 | 3.8 | >36 (33) | >36 (46) | (21) | No | Trichimeric | Alive | (36) | ||
| G664 | 2.8 | 2.0 | >28 (52) | 26 (0) | [R] | (48) | No | Bichimeric | Adopted | (28) | |
Fluorescent VNTR-PCR analysis was used to determine percent donor chimerism from 2 donors in a single recipient. Percent chimerism was determined for the week indicated for the duration of donor 1 and donor 2 engraftment. G551 long-term trichimerism was confirmed at week 37 using bone marrow cells. GVHD was established by serially following clinical signs and symptoms and liver enzyme chemistries in peripheral blood of the recipient and tissue biopsy histological confirmation. GVHD was treated with CSP.
G631 had to be euthanized before donor 1 and donor 2 hematopoietic chimerism was completely rejected.
D1 indicates donor 1; D2, donor2; E1, euthanized at end of study; E2, euthanized, thrombocytopenia; and [R], rejection.