Table 1. Primers and probes used for... | American Society of Hematology

Table 1.

Primers and probes used for quantitative PCR analysis of endogenous and/or retroviral JAK2 mRNA

	Forward primers 5′-3′	Probes 6-FAM/5′-3′/TAMRA	Reverse primers 5′-3′
Retroviral JAK2	CGTCTCTCCCCCTTGAACCT	TTCGACCCCGCCTCGATCCTC	GAAGGAGTGAGGGCTGGATAAA
Total JAK2	TGTAACTGTCCATAAACAAGATGGTAAAG	TTTGGAGATAGAACTTAGCTCATTAAAAGAAGCCTTGTC	TACCCGTCAATTAATGACACGAA
Endogenous JAK2	GCCTTCACTCAGAGACCAAGCA	ACTTCCAGAACCAGAACAAAGCTCTGTAGCCT	CGTCACAGTTTCTTCTGCCTAGCT
Tubulin	GGGTGGAGGCACTGGCT	TGGCATGGGCACCCTGCTCAT	GTCAGGATATTCTTCCCGGATCT

	Forward primers 5′-3′	Probes 6-FAM/5′-3′/TAMRA	Reverse primers 5′-3′
Retroviral JAK2	CGTCTCTCCCCCTTGAACCT	TTCGACCCCGCCTCGATCCTC	GAAGGAGTGAGGGCTGGATAAA
Total JAK2	TGTAACTGTCCATAAACAAGATGGTAAAG	TTTGGAGATAGAACTTAGCTCATTAAAAGAAGCCTTGTC	TACCCGTCAATTAATGACACGAA
Endogenous JAK2	GCCTTCACTCAGAGACCAAGCA	ACTTCCAGAACCAGAACAAAGCTCTGTAGCCT	CGTCACAGTTTCTTCTGCCTAGCT
Tubulin	GGGTGGAGGCACTGGCT	TGGCATGGGCACCCTGCTCAT	GTCAGGATATTCTTCCCGGATCT