Heterozygosity and homozygosity at the lpr locus rescue the accelerated erythroid differentiation caused by lack of Raf-1
. | Genotype . | . | . | . | . | . | . | . | . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Differentiation cell type . | +/+ . | . | . | Ipr/+ . | . | . | Ipr/Ipr . | . | . | ||||||||
| . | Raf-1+/+ . | Raf-1+/− . | Raf-1−/− . | Raf-1+/+ . | Raf-1+/− . | Raf-1−/− . | Raf-1+/+ . | Raf-1+/− . | Raf-1−/− . | ||||||||
| 0 h | |||||||||||||||||
| PB | 83.0 | 83.0 | 80.0 | 77.8 | 82.0 | 76.8 | 94.0 | 87.0 | 81.1 | ||||||||
| DB | 8.6 | 14.7 | 14.4 | 18.7 | 8.0 | 18.2 | 2.0 | 9.3 | 13.0 | ||||||||
| E | 7.0 | 1.0 | 4.0 | 2.4 | 8.5 | 3.5 | 3.0 | 2.7 | 0.5 | ||||||||
| DC | 1.4 | 1.3 | 1.6 | 1.1 | 1.5 | 1.5 | 1.0 | 1.0 | 5.4 | ||||||||
| 24 h | |||||||||||||||||
| PB | 45.0 | 17.0 | 10.0 | 57.3 | 43.0 | 13.7 | 71.6 | 72.0 | 60.1 | ||||||||
| DB | 43.5 | 31.1 | 25.0 | 37.1 | 41.9 | 29.8 | 26.4 | 23.7 | 29.5 | ||||||||
| E | 10.0 | 50.2 | 63.7 | 4.4 | 13.4 | 55.4 | 1.1 | 3.1 | 5.2 | ||||||||
| DC | 1.5 | 1.7 | 1.3 | 1.2 | 1.7 | 1.1 | 0.9 | 1.2 | 5.2 | ||||||||
| 36 h | |||||||||||||||||
| PB | 16.7 | 2.7 | 0.5 | 21.4 | 16.7 | 1.7 | 57.0 | 55.0 | 7.0 | ||||||||
| DB | 30.0 | 17.9 | 9.0 | 58.6 | 35.0 | 17.5 | 37.6 | 40.2 | 79.8 | ||||||||
| E | 52.0 | 78.2 | 89.5 | 18.7 | 46.8 | 79.6 | 4.0 | 3.7 | 7.0 | ||||||||
| DC | 1.3 | 1.2 | 1.0 | 1.3 | 1.5 | 1.2 | 1.4 | 1.1 | 6.2 | ||||||||
| 48 h | |||||||||||||||||
| PB | 1.5 | 1.6 | 0.2 | 3.2 | 2.4 | 1.2 | 27.0 | 23.0 | 2.0 | ||||||||
| DB | 7.0 | 5.6 | 2.5 | 22.3 | 5.0 | 3.2 | 56.0 | 60.0 | 73.9 | ||||||||
| E | 90.0 | 91.3 | 95.6 | 73.4 | 91.0 | 94.2 | 15.5 | 15.6 | 17.0 | ||||||||
| DC | 1.5 | 1.5 | 1.7 | 1.1 | 1.6 | 1.4 | 1.5 | 1.4 | 7.1 | ||||||||
. | Genotype . | . | . | . | . | . | . | . | . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Differentiation cell type . | +/+ . | . | . | Ipr/+ . | . | . | Ipr/Ipr . | . | . | ||||||||
| . | Raf-1+/+ . | Raf-1+/− . | Raf-1−/− . | Raf-1+/+ . | Raf-1+/− . | Raf-1−/− . | Raf-1+/+ . | Raf-1+/− . | Raf-1−/− . | ||||||||
| 0 h | |||||||||||||||||
| PB | 83.0 | 83.0 | 80.0 | 77.8 | 82.0 | 76.8 | 94.0 | 87.0 | 81.1 | ||||||||
| DB | 8.6 | 14.7 | 14.4 | 18.7 | 8.0 | 18.2 | 2.0 | 9.3 | 13.0 | ||||||||
| E | 7.0 | 1.0 | 4.0 | 2.4 | 8.5 | 3.5 | 3.0 | 2.7 | 0.5 | ||||||||
| DC | 1.4 | 1.3 | 1.6 | 1.1 | 1.5 | 1.5 | 1.0 | 1.0 | 5.4 | ||||||||
| 24 h | |||||||||||||||||
| PB | 45.0 | 17.0 | 10.0 | 57.3 | 43.0 | 13.7 | 71.6 | 72.0 | 60.1 | ||||||||
| DB | 43.5 | 31.1 | 25.0 | 37.1 | 41.9 | 29.8 | 26.4 | 23.7 | 29.5 | ||||||||
| E | 10.0 | 50.2 | 63.7 | 4.4 | 13.4 | 55.4 | 1.1 | 3.1 | 5.2 | ||||||||
| DC | 1.5 | 1.7 | 1.3 | 1.2 | 1.7 | 1.1 | 0.9 | 1.2 | 5.2 | ||||||||
| 36 h | |||||||||||||||||
| PB | 16.7 | 2.7 | 0.5 | 21.4 | 16.7 | 1.7 | 57.0 | 55.0 | 7.0 | ||||||||
| DB | 30.0 | 17.9 | 9.0 | 58.6 | 35.0 | 17.5 | 37.6 | 40.2 | 79.8 | ||||||||
| E | 52.0 | 78.2 | 89.5 | 18.7 | 46.8 | 79.6 | 4.0 | 3.7 | 7.0 | ||||||||
| DC | 1.3 | 1.2 | 1.0 | 1.3 | 1.5 | 1.2 | 1.4 | 1.1 | 6.2 | ||||||||
| 48 h | |||||||||||||||||
| PB | 1.5 | 1.6 | 0.2 | 3.2 | 2.4 | 1.2 | 27.0 | 23.0 | 2.0 | ||||||||
| DB | 7.0 | 5.6 | 2.5 | 22.3 | 5.0 | 3.2 | 56.0 | 60.0 | 73.9 | ||||||||
| E | 90.0 | 91.3 | 95.6 | 73.4 | 91.0 | 94.2 | 15.5 | 15.6 | 17.0 | ||||||||
| DC | 1.5 | 1.5 | 1.7 | 1.1 | 1.6 | 1.4 | 1.5 | 1.4 | 7.1 | ||||||||
Erythroid progenitors of the indicated genotypes were cultured in differentiation medium. At the indicated times, cytospins stained with neutral benzidine plus histologic dyes were examined to determine the progress of differentiation. The values represent the mean of 4 determinations, each carried out by an independent investigator evaluating 500 cells per sample on at least 2 randomly selected fields.
PB indicates proliferating blast; DB, differentiating blast; E, mature erythrocytes; DC, apoptotic/dead cells.