Influence of the cytokine environment on the FcγRIIa/FcγRIIb2 mRNA ratio in neutrophils and PBMCs
. | FcγRIIa/FcγRIIb2 mRNA ratio . | . | . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|
. | No stimulus . | GM-CSF, 50 U/mL . | LPS, 20 ng/mL . | IFNγ, 200 U/mL . | IL-4, 200 ng/mL . | ||||
| Donor 1 | |||||||||
| Neutrophils | 0.9 ± 0.04 | 0.9 ± 0.04 | 0.8 ± 0.08 | 0.9 ± 0.00 | 0.9 ± 0.00 | ||||
| PBMCs | 0.7 ± 0.12 | 0.2 ± 0.00 | 0.9 ± 0.03 | 0.7 ± 0.04 | 0.1 ± 0.00 | ||||
| Donor 2 | |||||||||
| Neutrophils | 0.9 ± 0.01 | 0.8 ± 0.00 | 0.9 ± 0.02 | 0.9 ± 0.05 | 0.9 ± 0.02 | ||||
| PBMCs | 0.7 ± 0.04 | 0.2 ± 0.02 | 0.5 ± 0.03 | 0.6 ± 0.02 | 0.1 ± 0.00 | ||||
| Donor 3 | |||||||||
| Neutrophils | 1.9 ± 0.02 | 2.0 ± 0.06 | 2.2 ± 0.07 | 1.9 ± 0.06 | 1.9 ± 0.07 | ||||
| PBMCs | 0.6 ± 0.02 | 0.1 ± 0.00 | 0.5 ± 0.01 | 0.4 ± 0.01 | 0.1 ± 0.00 | ||||
| Donor 4 | |||||||||
| Neutrophils | 1.9 ± 0.03 | 1.7 ± 0.02 | 1.9 ± 0.01 | 1.7 ± 0.01 | 1.9 ± 0.00 | ||||
| PBMCs | 1.6 ± 0.07 | 0.4 ± 0.01 | 1.1 ± 0.13 | 1.5 ± 0.07 | 0.2 ± 0.00 | ||||
. | FcγRIIa/FcγRIIb2 mRNA ratio . | . | . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|
. | No stimulus . | GM-CSF, 50 U/mL . | LPS, 20 ng/mL . | IFNγ, 200 U/mL . | IL-4, 200 ng/mL . | ||||
| Donor 1 | |||||||||
| Neutrophils | 0.9 ± 0.04 | 0.9 ± 0.04 | 0.8 ± 0.08 | 0.9 ± 0.00 | 0.9 ± 0.00 | ||||
| PBMCs | 0.7 ± 0.12 | 0.2 ± 0.00 | 0.9 ± 0.03 | 0.7 ± 0.04 | 0.1 ± 0.00 | ||||
| Donor 2 | |||||||||
| Neutrophils | 0.9 ± 0.01 | 0.8 ± 0.00 | 0.9 ± 0.02 | 0.9 ± 0.05 | 0.9 ± 0.02 | ||||
| PBMCs | 0.7 ± 0.04 | 0.2 ± 0.02 | 0.5 ± 0.03 | 0.6 ± 0.02 | 0.1 ± 0.00 | ||||
| Donor 3 | |||||||||
| Neutrophils | 1.9 ± 0.02 | 2.0 ± 0.06 | 2.2 ± 0.07 | 1.9 ± 0.06 | 1.9 ± 0.07 | ||||
| PBMCs | 0.6 ± 0.02 | 0.1 ± 0.00 | 0.5 ± 0.01 | 0.4 ± 0.01 | 0.1 ± 0.00 | ||||
| Donor 4 | |||||||||
| Neutrophils | 1.9 ± 0.03 | 1.7 ± 0.02 | 1.9 ± 0.01 | 1.7 ± 0.01 | 1.9 ± 0.00 | ||||
| PBMCs | 1.6 ± 0.07 | 0.4 ± 0.01 | 1.1 ± 0.13 | 1.5 ± 0.07 | 0.2 ± 0.00 | ||||
Purified neutrophils and PBMC fractions were each stimulated in triplicate for 4 hours with the stimulants indicated. Data are presented as means ± SEM.