Table 1.

Gene expression patterns of in vivo—elicited M2

Expression in M2
Genes up-regulated in M2 in the PLC-/-Trypanosoma brucei brucei model*
PCR primer sequences, 5′-3′
GenBank accession no.
Tco†
Tcr‡
Sp3§
Cluster 1      
    Arginase-1   GCTGTCTTCCCAAGAGTTGGG  NM_007482  I   I   I  
  ATGGAAGAGACCTTCAGCTAC      
    Cathepsin D   CCTTCGCGATTATCAGAATCC  NM_009983  NI   I   I  
  TACTTATGGTGGACCCAGCA      
    CD163  GAGCATGAATGAAGTGTCCG  NM_053094  ND   NI   I  
  TGCTGAAGTTGTCGTCACAC      
    Coagulation factor XIIIA (F13a)  CCAGGAATTAAGCAAGACATC  NM_028784  NI   I   I  
  TGCCCTTACTTCTAGTTCTC      
    Found in inflammatory zone 1 (Fizz1; resistin-like alpha)   TCCCAGTGAATACTGATGAGA  NM_020509  I   I   I  
  CCACTCTGGATCTCCCAAGA      
    NGF-beta  CATAGCGTAATGTCCATGTTGTTCT  NM_013609  I   NI   NI  
  CTTCTCATCTGTTGTCAACGC      
    Pafah  TGCACCAGAACTTTGACGAC  NM_013737  I   I   I  
  CAACTGTGTAACAAGCAAGTTC      
    Prosaposin   GCCATTGTCATAGCACAGAG  NM_011179  I   I   I  
  CTTCACTCATGAGGTGAACAG      
    Sep  TTCTGCAGGCATCCAGATTG  NM_009155  I   I   I  
  CACAAGACGGCCACATCTGT      
    Trem2  CTCCGAGGCCGAGAGGC  NM_031254  I   I   I  
  GGGCACCCTCGAAACTCGA      
Cluster 2      
    Cullin3   GATTCTTACCAAGTCCAGTTG  NM_016716  I   NI   I  
  GAAGCTCAATCAACTGATGTTG      
    Epithelial membrane protein 1 (Emp1)  GAGTGGTCTCCTCTCAACAG  NM_010128  NI   I   I  
  AGCTACCATCTGTCTGGATG      
    Gas3  GTAATGGACACACGACTGATC  NM_008885  I   I   I  
  GGAGTAGTCAGTGTTGACATG      
    Longevity assurance homolog 2 (Lass2)  AACAGCCTATCTCCAGGTAG  NM_029789  NI   NI   NI  
  GCTTAGAAAGATGGAACCTTG      
Cluster 3      
    Mgl1  ATGATGTCTGCCAGAGAACC  NM_010796  I   I   I  
  ATCACAGATTTCAGCAACCTTA      
    Mgl2  GATAACTGGCATGGACATATG  NM_145137  I   I   I  
  TTTCTAATCACCATAACACATTC      
    Mmr  GCAAATGGAGCCGTCTGTGC  NM_008625  I   I   I  
  CTCGTGGATCTCCGTGACAC      
    Macrophage-inducible C-type lectin (Mincle)  ACACAGAGAGAGGATGCTTC  NM_019948  NI   I   NI  
  TGTCGATAACCACCAGGTGA      
    Ym(1/2)  GGGCATACCTTTATCCTGAG  NM_009892/NM_145126   I   I   I  
  CCACTGAAGTCATCCATGTC      
Cluster 4      
    Aldehyde reductase   TATGGCAATGAAACTGAGATTG  NM_021473  NI   I   I  
  CAGGTCTCTTTATAGTGAGTTG      
    Ankycorbin   GTGCAGAAGTTCCAGCGAG  NM_030690  NI   NI   I  
  ATGCATTCTGTAAACTGAGATG      
    E-cadherin   ACTTGGGGACAGCAACATCA  NM_009864  I   I   I  
  GGGTTTAAATCGGCCAGCAT      
    Cathepsin B   AGAGGTGTGTCATATGTAGAC  NM_007798  NI   I   NI  
  GACTACATAAGACACTGTCTC      
    Cathepsin L   TGGACTCGGAGGAGTCTTAC  NM_009984  NI   I   I  
  TCTGTTCCTTCATAGCCATAG      
    Cathepsin S   AGAAGGGCTGCGTCACTGAG  NM_021281  NI   I   I  
  GAATGTACCTTGAACACGTAG      
    Cationic amino acid transporter 2B   GTACTCATAAGGTTTTGTAGAAG  NM_007514  NI   I   I  
  GTACCCACACCTCGTCCCTAG      
    Folate receptor 2 (Folr2)  GGAGCTACACAAGGCTGAC  NM_008035  I   I   I  
  TGTGACAGGGTGCTGTGTTT      
    Lysosomal-associated membrane protein 2 (Lamp2)  AGGAGTACTTATTCTAGTGTTG  NM_010685  NI   I   I  
  CTGCATTTTAAGCAGATCAGAG      
    Leucine-rich alpha-2 glycoprotein 1 (Lrg1)  AACTGCTTTGGTGACCCCTGA  NM_029796  I   NI   I  
  GCCATGAAGGGTCAGCGGCTG      
    Ten-cysteine protein 1 (Xcp1; resistin-like gamma)   CTTGCCAATCGAGATGACTG  NM_181596  NI   I   NI  
 		 GGACAATATGAATTCATCAATG
 
 
 
 
 
Expression in M2
Genes up-regulated in M2 in the PLC-/-Trypanosoma brucei brucei model*
PCR primer sequences, 5′-3′
GenBank accession no.
Tco†
Tcr‡
Sp3§
Cluster 1      
    Arginase-1   GCTGTCTTCCCAAGAGTTGGG  NM_007482  I   I   I  
  ATGGAAGAGACCTTCAGCTAC      
    Cathepsin D   CCTTCGCGATTATCAGAATCC  NM_009983  NI   I   I  
  TACTTATGGTGGACCCAGCA      
    CD163  GAGCATGAATGAAGTGTCCG  NM_053094  ND   NI   I  
  TGCTGAAGTTGTCGTCACAC      
    Coagulation factor XIIIA (F13a)  CCAGGAATTAAGCAAGACATC  NM_028784  NI   I   I  
  TGCCCTTACTTCTAGTTCTC      
    Found in inflammatory zone 1 (Fizz1; resistin-like alpha)   TCCCAGTGAATACTGATGAGA  NM_020509  I   I   I  
  CCACTCTGGATCTCCCAAGA      
    NGF-beta  CATAGCGTAATGTCCATGTTGTTCT  NM_013609  I   NI   NI  
  CTTCTCATCTGTTGTCAACGC      
    Pafah  TGCACCAGAACTTTGACGAC  NM_013737  I   I   I  
  CAACTGTGTAACAAGCAAGTTC      
    Prosaposin   GCCATTGTCATAGCACAGAG  NM_011179  I   I   I  
  CTTCACTCATGAGGTGAACAG      
    Sep  TTCTGCAGGCATCCAGATTG  NM_009155  I   I   I  
  CACAAGACGGCCACATCTGT      
    Trem2  CTCCGAGGCCGAGAGGC  NM_031254  I   I   I  
  GGGCACCCTCGAAACTCGA      
Cluster 2      
    Cullin3   GATTCTTACCAAGTCCAGTTG  NM_016716  I   NI   I  
  GAAGCTCAATCAACTGATGTTG      
    Epithelial membrane protein 1 (Emp1)  GAGTGGTCTCCTCTCAACAG  NM_010128  NI   I   I  
  AGCTACCATCTGTCTGGATG      
    Gas3  GTAATGGACACACGACTGATC  NM_008885  I   I   I  
  GGAGTAGTCAGTGTTGACATG      
    Longevity assurance homolog 2 (Lass2)  AACAGCCTATCTCCAGGTAG  NM_029789  NI   NI   NI  
  GCTTAGAAAGATGGAACCTTG      
Cluster 3      
    Mgl1  ATGATGTCTGCCAGAGAACC  NM_010796  I   I   I  
  ATCACAGATTTCAGCAACCTTA      
    Mgl2  GATAACTGGCATGGACATATG  NM_145137  I   I   I  
  TTTCTAATCACCATAACACATTC      
    Mmr  GCAAATGGAGCCGTCTGTGC  NM_008625  I   I   I  
  CTCGTGGATCTCCGTGACAC      
    Macrophage-inducible C-type lectin (Mincle)  ACACAGAGAGAGGATGCTTC  NM_019948  NI   I   NI  
  TGTCGATAACCACCAGGTGA      
    Ym(1/2)  GGGCATACCTTTATCCTGAG  NM_009892/NM_145126   I   I   I  
  CCACTGAAGTCATCCATGTC      
Cluster 4      
    Aldehyde reductase   TATGGCAATGAAACTGAGATTG  NM_021473  NI   I   I  
  CAGGTCTCTTTATAGTGAGTTG      
    Ankycorbin   GTGCAGAAGTTCCAGCGAG  NM_030690  NI   NI   I  
  ATGCATTCTGTAAACTGAGATG      
    E-cadherin   ACTTGGGGACAGCAACATCA  NM_009864  I   I   I  
  GGGTTTAAATCGGCCAGCAT      
    Cathepsin B   AGAGGTGTGTCATATGTAGAC  NM_007798  NI   I   NI  
  GACTACATAAGACACTGTCTC      
    Cathepsin L   TGGACTCGGAGGAGTCTTAC  NM_009984  NI   I   I  
  TCTGTTCCTTCATAGCCATAG      
    Cathepsin S   AGAAGGGCTGCGTCACTGAG  NM_021281  NI   I   I  
  GAATGTACCTTGAACACGTAG      
    Cationic amino acid transporter 2B   GTACTCATAAGGTTTTGTAGAAG  NM_007514  NI   I   I  
  GTACCCACACCTCGTCCCTAG      
    Folate receptor 2 (Folr2)  GGAGCTACACAAGGCTGAC  NM_008035  I   I   I  
  TGTGACAGGGTGCTGTGTTT      
    Lysosomal-associated membrane protein 2 (Lamp2)  AGGAGTACTTATTCTAGTGTTG  NM_010685  NI   I   I  
  CTGCATTTTAAGCAGATCAGAG      
    Leucine-rich alpha-2 glycoprotein 1 (Lrg1)  AACTGCTTTGGTGACCCCTGA  NM_029796  I   NI   I  
  GCCATGAAGGGTCAGCGGCTG      
    Ten-cysteine protein 1 (Xcp1; resistin-like gamma)   CTTGCCAATCGAGATGACTG  NM_181596  NI   I   NI  
 		 GGACAATATGAATTCATCAATG
 
 
 
 
 

Genes were tentatively grouped in clusters based on documented functional properties of the gene products. Cluster 1 includes anti-inflammation, wound healing, and angiogenesis; cluster 2, cell-cycle regulation; cluster 3, lectins; and cluster 4, miscellaneous. Gene expression levels were determined by reverse transcription followed by real-time quantitative PCR and were normalized with the housekeeping gene ribosomal protein S12. The first and second sequences of each primer pair represent sense and antisense, respectively, except for arginase-1, CAT2B, and Lrg1, where the first primer is antisense, and the second is sense.

I indicates significantly induced (P < .05); NI, not significantly induced; ND, expression not detectable.

*

Genes significantly (P < .05) up-regulated in peritoneal M2 compared with M1 cells elicited during PLC-/-Trypanosoma brucei brucei infection.

†

Expression in splenic M2 compared with M1 during Trypanosoma congolense infection.

‡

Expression in peritoneal M2 during Taenia crassiceps infection compared with peritoneal myeloid cells from noninfected animals.

§

Expression in splenic M2 cells compared with M1 cells in the BW-Sp3 tumor model.

View Large
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal