Tumor-associated macrophages are M2 oriented
M2 marker genes . | Tumor/peritoneal macrophages . |
---|---|
FIZZ1 | 16.3 ± 6.1 |
Ym | 9.9 ± 3.6 |
Fibronectin-1 | 4.8 ± 1.5 |
Arginase-1 | 21.3 ± 9.7 |
PPARγ | 8.9 ± 4.6 |
M2 marker genes . | Tumor/peritoneal macrophages . |
---|---|
FIZZ1 | 16.3 ± 6.1 |
Ym | 9.9 ± 3.6 |
Fibronectin-1 | 4.8 ± 1.5 |
Arginase-1 | 21.3 ± 9.7 |
PPARγ | 8.9 ± 4.6 |
Tumor-associated and peritoneal macrophages from 2 tumor-bearers were obtained via magnetic sorting using anti-CD11b microbeads, after which RNA was extracted and cDNA prepared from these samples. Expression of each of the listed genes was determined via quantitative real-time PCR and normalized for the housekeeping gene ribosomal protein S12. The fold induction of gene expression (mean ± SD of 2 individual mice) in tumor-associated macrophages compared with peritoneal macrophages (mean ± SD of 2 individual mice) is shown.