Table 3.

Tumor-associated macrophages are M2 oriented


M2 marker genes

Tumor/peritoneal macrophages
FIZZ1   16.3 ± 6.1  
Ym   9.9 ± 3.6  
Fibronectin-1   4.8 ± 1.5  
Arginase-1   21.3 ± 9.7  
PPARγ
 
8.9 ± 4.6
 

M2 marker genes

Tumor/peritoneal macrophages
FIZZ1   16.3 ± 6.1  
Ym   9.9 ± 3.6  
Fibronectin-1   4.8 ± 1.5  
Arginase-1   21.3 ± 9.7  
PPARγ
 
8.9 ± 4.6
 

Tumor-associated and peritoneal macrophages from 2 tumor-bearers were obtained via magnetic sorting using anti-CD11b microbeads, after which RNA was extracted and cDNA prepared from these samples. Expression of each of the listed genes was determined via quantitative real-time PCR and normalized for the housekeeping gene ribosomal protein S12. The fold induction of gene expression (mean ± SD of 2 individual mice) in tumor-associated macrophages compared with peritoneal macrophages (mean ± SD of 2 individual mice) is shown.

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