Coagulation factor activity in C-FFP and PCT-FFP
Factor . | C-FFP . | PCT-FFP . | Reference range* . |
|---|---|---|---|
| Fibrinogen level, g/L | 2.72 ± 0.30 | 2.18 ± 0.47 | 1.83-4.03 |
| Factor II level, U/mL | 0.94 ± 0.15 | 0.91 ± 0.13 | 0.79-1.37 |
| Factor V level, U/mL | 1.31 ± 0.27 | 1.09 ± 0.18 | 0.82-1.60 |
| Factor VII level, U/mL | 1.34 ± 0.27 | 0.92 ± 0.24 | 0.66-1.80 |
| Factor VIII level, U/mL | 1.65 ± 0.27 | 1.17 ± 0.34 | 0.73-2.53 |
| Factor IX level, U/mL | 1.15 ± 0.11 | 0.93 ± 0.19 | 0.68-1.50 |
| Factor X level, U/mL | 0.95 ± 0.9 | 0.89 ± 0.16 | 0.73-1.36 |
| Factor XI level, U/mL | 0.92 ± 0.17 | 0.94 ± 0.18 | 0.71-1.56 |
| Factor XIII level, U/mL | 1.08 ± 0.11 | 1.07 ± 0.11 | 0.65-1.65 |
Factor . | C-FFP . | PCT-FFP . | Reference range* . |
|---|---|---|---|
| Fibrinogen level, g/L | 2.72 ± 0.30 | 2.18 ± 0.47 | 1.83-4.03 |
| Factor II level, U/mL | 0.94 ± 0.15 | 0.91 ± 0.13 | 0.79-1.37 |
| Factor V level, U/mL | 1.31 ± 0.27 | 1.09 ± 0.18 | 0.82-1.60 |
| Factor VII level, U/mL | 1.34 ± 0.27 | 0.92 ± 0.24 | 0.66-1.80 |
| Factor VIII level, U/mL | 1.65 ± 0.27 | 1.17 ± 0.34 | 0.73-2.53 |
| Factor IX level, U/mL | 1.15 ± 0.11 | 0.93 ± 0.19 | 0.68-1.50 |
| Factor X level, U/mL | 0.95 ± 0.9 | 0.89 ± 0.16 | 0.73-1.36 |
| Factor XI level, U/mL | 0.92 ± 0.17 | 0.94 ± 0.18 | 0.71-1.56 |
| Factor XIII level, U/mL | 1.08 ± 0.11 | 1.07 ± 0.11 | 0.65-1.65 |
Coagulation factor activity was measured using standard coagulation assays for retained samples of C-FFP and PCT-FFP taken from 274 to 277 randomly selected units and stored for variable periods during the study. The mean volume of PCT-FFP units was 285 ± 0.3 mL (range, 280 to 289 mL). Samples for coagulation assays were obtained after preparation and prior to freezing. Aliquots were stored at –18°C and assayed during the course of the study at approximately the same time that full units were transfused. Approximately 9000 PCT-FFP units were prepared during the study. The functional activities (mean ± SD) for coagulation factor proteins were calculated and compared with a reference range for C-FFP sampled before freezing and stored for variable periods. Factor XIII activity was based on analysis of 14 C-FFP and PCT-FFP units.
Reference ranges for C-FFP were determined using samples obtained from plasma units (n = 274 to 277) obtained after collection and prior to PCT processing and freezing. The reference range was defined as the 95% central interval for coagulation factor activity in plasma derived from CPD-A whole blood using approved collection systems (Transfusion Therapies, Baxter Healthcare, Deerfield, IL)