Movements of transmigrated neutrophils, PBLs, and monocytes beneath the EC monolayers under VSF and static condition
. | a . | b . | c . | d . | Static . |
|---|---|---|---|---|---|
| Total distance (T), μm | |||||
| Neutrophils | 252.63 ± 20.06 | 277.52 ± 12.13 | 183.67 ± 11.60* | 256.80 ± 13.75 | 233.66 ± 9.86 |
| PBLs | 89.93 ± 4.40 | 125.07 ± 14.79 | 50.73 ± 3.61* | 116.62 ± 7.84 | 69.77 ± 3.31 |
| Monocytes | 21.82 ± 0.78 | 24.51 ± 1.54 | 10.62 ± 1.08* | 24.28 ± 1.66 | 20.14 ± 1.41 |
| Distance from initial to final position (N), μm | |||||
| Neutrophils | 52.47 ± 6.35 | 60.01 ± 7.10 | 23.27 ± 2.92* | 75.93 ± 6.96 | 43.04 ± 4.45 |
| PBLs | 82.46 ± 4.72 | 121.13 ± 14.32 | 40.07 ± 3.76* | 111.53 ± 7.58 | 50.07 ± 2.49 |
| Monocytes | 14.86 ± 0.55 | 17.74 ± 0.87 | 8.12 ± 0.32* | 16.95 ± 1.14 | 13.76 ± 1.43 |
| Randomness, T/N | |||||
| Neutrophils | 5.27 ± 0.42 | 5.87 ± 0.88 | 10.86 ± 1.92* | 3.98 ± 0.47 | 6.25 ± 0.55 |
| PBLs | 1.12 ± 0.09 | 1.03 ± 0.02 | 1.46 ± 0.25 | 1.05 ± 0.01 | 1.41 ± 0.07 |
| Monocytes | 1.31 ± 0.05 | 1.34 ± 0.07 | 1.12 ± 0.02* | 1.26 ± 0.04 | 1.57 ± 0.11 |
. | a . | b . | c . | d . | Static . |
|---|---|---|---|---|---|
| Total distance (T), μm | |||||
| Neutrophils | 252.63 ± 20.06 | 277.52 ± 12.13 | 183.67 ± 11.60* | 256.80 ± 13.75 | 233.66 ± 9.86 |
| PBLs | 89.93 ± 4.40 | 125.07 ± 14.79 | 50.73 ± 3.61* | 116.62 ± 7.84 | 69.77 ± 3.31 |
| Monocytes | 21.82 ± 0.78 | 24.51 ± 1.54 | 10.62 ± 1.08* | 24.28 ± 1.66 | 20.14 ± 1.41 |
| Distance from initial to final position (N), μm | |||||
| Neutrophils | 52.47 ± 6.35 | 60.01 ± 7.10 | 23.27 ± 2.92* | 75.93 ± 6.96 | 43.04 ± 4.45 |
| PBLs | 82.46 ± 4.72 | 121.13 ± 14.32 | 40.07 ± 3.76* | 111.53 ± 7.58 | 50.07 ± 2.49 |
| Monocytes | 14.86 ± 0.55 | 17.74 ± 0.87 | 8.12 ± 0.32* | 16.95 ± 1.14 | 13.76 ± 1.43 |
| Randomness, T/N | |||||
| Neutrophils | 5.27 ± 0.42 | 5.87 ± 0.88 | 10.86 ± 1.92* | 3.98 ± 0.47 | 6.25 ± 0.55 |
| PBLs | 1.12 ± 0.09 | 1.03 ± 0.02 | 1.46 ± 0.25 | 1.05 ± 0.01 | 1.41 ± 0.07 |
| Monocytes | 1.31 ± 0.05 | 1.34 ± 0.07 | 1.12 ± 0.02* | 1.26 ± 0.04 | 1.57 ± 0.11 |
Purified neutrophils, PBLs, or monocytes were perfused over the EC/SMC cocultures, and the subendothelial migration of arrested WBCs was traced for 20 minutes in areas a, b, c, and d under VSF or under static conditions after their transmigration across the EC monolayer to its underside. The distance traversed by the cell center was measured every 20 seconds and summed for each cell as the total migration distance (T) in 20 minutes. The net distance traversed by the cell center (N) was determined from the displacement between the initial (0 minute) and final (20 minutes) position, and the T/N ratio was calculated as an index of randomness of cell movement. Data shown are mean ± SEM of 25 cells from 3 independent experiments.
P < .05 for comparison of area c versus areas a, b, and d and static