Rolling velocities and transmigration times of neutrophils, PBLs, and monocytes on ECs cocultured with SMCs under VSF
. | a . | b . | c . | d . | Static . |
|---|---|---|---|---|---|
| Average rolling velocity, μm/sec | |||||
| Neutrophils | 7.15 ± 0.52 | 11.37 ± 0.46 | 5.72 ± 0.20* | 12.04 ± 0.34 | ND |
| PBLs | 7.03 ± 0.28 | 12.01 ± 0.36 | 5.18 ± 0.27* | 12.54 ± 0.41 | ND |
| Monocytes | 8.41 ± 0.22 | 10.66 ± 0.32 | 4.63 ± 0.34* | 11.57 ± 0.31 | ND |
| Transmigration time, sec | |||||
| Neutrophils | 100.34 ± 4.69 | 95.77 ± 5.79 | 58.27 ± 2.13* | 99.75 ± 4.88 | 103.86 ± 5.10 |
| PBLs | 195.21 ± 7.56 | 173.73 ± 8.52 | 131.21 ± 8.11* | 177.53 ± 7.63 | 183.73 ± 7.31 |
| Monocytes | 313.08 ± 8.49 | 293.83 ± 7.90 | 204.40 ± 7.38* | 291.06 ± 8.10 | 337.92 ± 7.83 |
. | a . | b . | c . | d . | Static . |
|---|---|---|---|---|---|
| Average rolling velocity, μm/sec | |||||
| Neutrophils | 7.15 ± 0.52 | 11.37 ± 0.46 | 5.72 ± 0.20* | 12.04 ± 0.34 | ND |
| PBLs | 7.03 ± 0.28 | 12.01 ± 0.36 | 5.18 ± 0.27* | 12.54 ± 0.41 | ND |
| Monocytes | 8.41 ± 0.22 | 10.66 ± 0.32 | 4.63 ± 0.34* | 11.57 ± 0.31 | ND |
| Transmigration time, sec | |||||
| Neutrophils | 100.34 ± 4.69 | 95.77 ± 5.79 | 58.27 ± 2.13* | 99.75 ± 4.88 | 103.86 ± 5.10 |
| PBLs | 195.21 ± 7.56 | 173.73 ± 8.52 | 131.21 ± 8.11* | 177.53 ± 7.63 | 183.73 ± 7.31 |
| Monocytes | 313.08 ± 8.49 | 293.83 ± 7.90 | 204.40 ± 7.38* | 291.06 ± 8.10 | 337.92 ± 7.83 |
Purified neutrophils, PBLs, and monocytes were perfused over ECs/SMCs under VSF for 20 minutes, and video records were made of the adhesive behaviors in areas a, b, c, and d. The rolling velocity and transmigration time of purified WBCs in different areas were measured by computerized image analysis. Examples for areas b and c are provided in Video S2. The WBC rolling velocity was calculated by dividing the rolling length by the time elapsed every 10 seconds and then averaged. Only WBCs that rolled without stopping during the entire 10-second period were included in this analysis. The transmigration time of WBCs was measured by following individual cells from the time they firmly attached on the EC monolayer (bright appearance on phase microscopy) until they turned phase dark in appearance after passing through the EC monolayer. The results shown are mean ± SEM of 25 cells from 3 independent experiments.
ND indicates no rolling velocity detected.
P < .05 for comparison of area c versus areas a, b, and d