Table 1. Chimerism in primary sheep that... | American Society of Hematology

Table 1.

Chimerism in primary sheep that received transplants of hESC-derived HSCs

				Chimerism in BM by PCR, %^*(time, mo)	Chimerism by FACS, % (time, mo)†
Animal no.	No. H1 passages	Cell type	No. cells	Chimerism in BM by PCR, %^*(time, mo)		BM	PB
1748	56	CD34⁺/Lin^-	140 000	0.05 (39)	0.1 (10)	0.1 (10)
1749	56	CD34⁺/Lin^-	140 000	0 (39)	0.1 (10)	0.1 (10)
1757	56	CD34⁺/Lin^-	100 000	+‡ (6)	0 (5)	0.1 (5)
1838	35	CD34⁺/CD38^-	95 000	0.04 (35)	0 (7)	0 (7)
1871	45	CD34⁺/CD38^-	26 500	0 (34)	0 (6)	0 (6)
1885	48	CD34⁺/CD38^-	63 000	0.09 (33)	0.1 (17)	0.2 (17)
1886	49	CD34⁺/CD38^-	70 000	0.001 (33)	0.1 (17)	0.1 (17)
1887	49	CD34⁺/CD38^-	70 000	0.001 (33)	0 (17)	0.1 (17)

				Chimerism in BM by PCR, %^*(time, mo)	Chimerism by FACS, % (time, mo)†
Animal no.	No. H1 passages	Cell type	No. cells	Chimerism in BM by PCR, %^*(time, mo)		BM	PB
1748	56	CD34⁺/Lin^-	140 000	0.05 (39)	0.1 (10)	0.1 (10)
1749	56	CD34⁺/Lin^-	140 000	0 (39)	0.1 (10)	0.1 (10)
1757	56	CD34⁺/Lin^-	100 000	+‡ (6)	0 (5)	0.1 (5)
1838	35	CD34⁺/CD38^-	95 000	0.04 (35)	0 (7)	0 (7)
1871	45	CD34⁺/CD38^-	26 500	0 (34)	0 (6)	0 (6)
1885	48	CD34⁺/CD38^-	63 000	0.09 (33)	0.1 (17)	0.2 (17)
1886	49	CD34⁺/CD38^-	70 000	0.001 (33)	0.1 (17)	0.1 (17)
1887	49	CD34⁺/CD38^-	70 000	0.001 (33)	0 (17)	0.1 (17)

FACS indicates fluorescence-activated cell sorter.

Real-time quantitative PCR was carried out for human microsatellite DNA (Supplemental Methods) on BM samples at various periods after transplantation. PCR data for PB samples at the matching time points were all negative. Analyses were repeated at least once to confirm data reported. Minimal detection limit = 0.0001%

†

Flow cytometry analyses for human CD34 or CD45 was carried out at various periods after transplantation. Twenty thousand to 50 000 events were acquired for a single analysis per animal at the noted time point

‡

Animal no. 1757 was analyzed by PCR for human GAPDH (Figure S1)

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