Summary of the quantitative analyses of fMLP-stimulated WT and Lsc KO neutrophil morphology and migration
. | WT, mean ± SEM (n = 60 cells) . | Lsc KO, mean ± SEM (n = 60 cells) . | P . |
|---|---|---|---|
| Area, μm2 | 278.7 ± 9.7 | 253.7 ± 7.5 | < .05 |
| (+) Δ Area, μm2/15 min | 686.7 ± 30.0 | 1036.8 ± 38.4 | < .001 |
| (-) Δ Area, μm2/15 min | -721.6 ± 26.0 | -1049.4 ± 39.6 | < .001 |
| Perimeter, μm | 68.0 ± 1.5 | 69.6 ± 1.3 | NS |
| (+) Δ Perimeter, μm/15 min | 116.5 ± 3.8 | 226.8 ± 24.5 | < .001 |
| (-) Δ Perimeter, μm/15 min | -123.4 ± 4.1 | -229.9 ± 26.9 | < .001 |
| Roundness, % | 76.4 ± 1.1 | 66.9 ± 1.0 | < .001 |
| (+) Δ Roundness, %/15 min | 176.2 ± 6.4 | 237.1 ± 7.9 | < .001 |
| (-) Δ Roundness, %/15 min | -171.5 ± 6.2 | -239.4 ± 8.7 | < .001 |
| Speed, μm/min | 4.7 ± 0.4 | 9.9 ± 0.4 | < .001 |
| Directionality, μm/μm | 0.45 ± 0.03 | 0.34 ± 0.02 | < .001 |
| Angle per turn, degrees | 48.8 ± 1.2 | 62.9 ± 1.0 | < .001 |
| Turns less than or equal to 30°/15 min | 29.1 ± 0.8 | 20.8 ± 0.5 | < .001 |
| Turns greater than 30°/15 min | 29.9 ± 0.8 | 38.2 ± 0.5 | < .001 |
. | WT, mean ± SEM (n = 60 cells) . | Lsc KO, mean ± SEM (n = 60 cells) . | P . |
|---|---|---|---|
| Area, μm2 | 278.7 ± 9.7 | 253.7 ± 7.5 | < .05 |
| (+) Δ Area, μm2/15 min | 686.7 ± 30.0 | 1036.8 ± 38.4 | < .001 |
| (-) Δ Area, μm2/15 min | -721.6 ± 26.0 | -1049.4 ± 39.6 | < .001 |
| Perimeter, μm | 68.0 ± 1.5 | 69.6 ± 1.3 | NS |
| (+) Δ Perimeter, μm/15 min | 116.5 ± 3.8 | 226.8 ± 24.5 | < .001 |
| (-) Δ Perimeter, μm/15 min | -123.4 ± 4.1 | -229.9 ± 26.9 | < .001 |
| Roundness, % | 76.4 ± 1.1 | 66.9 ± 1.0 | < .001 |
| (+) Δ Roundness, %/15 min | 176.2 ± 6.4 | 237.1 ± 7.9 | < .001 |
| (-) Δ Roundness, %/15 min | -171.5 ± 6.2 | -239.4 ± 8.7 | < .001 |
| Speed, μm/min | 4.7 ± 0.4 | 9.9 ± 0.4 | < .001 |
| Directionality, μm/μm | 0.45 ± 0.03 | 0.34 ± 0.02 | < .001 |
| Angle per turn, degrees | 48.8 ± 1.2 | 62.9 ± 1.0 | < .001 |
| Turns less than or equal to 30°/15 min | 29.1 ± 0.8 | 20.8 ± 0.5 | < .001 |
| Turns greater than 30°/15 min | 29.9 ± 0.8 | 38.2 ± 0.5 | < .001 |
(+) or (-) Δ Area, Δ perimeter, Δ roundness indicate sum of all increases (+) or sum of all decreases (-) in area, perimeter, or roundness, respectively, between successive frames (15-second intervals) during 15-minute experiment. Directionality (μm/μm) is net distance traveled/total linear distance traveled during 15-minute experiment.
NS indicates not significant.