Quantitative estimation of cell division upon homeostatic proliferation
. | CD8 T cells, day 7 . | . | CD4 T cells, day 17 . | . | ||
|---|---|---|---|---|---|---|
| Point of cell division . | Wild type . | c-myc+/- . | Wild type . | c-myc+/- . | ||
| Undivided | 31.4 ± 4.5 | 56.4 ± 2.4 | 45.5 ± 2.2 | 45.1 ± 2.2 | ||
| At first peak | 37.3 ± 2.2 | 29.2 ± 1.3 | 39.9 ± 0.1 | 42.7 ± 0.6 | ||
| At second peak | 17.7 ± 1.8 | 10.7 ± 2.3 | 11.4 ± 0.8 | 10.0 ± 1.0 | ||
| At third peak | 7.1 ± 0.6 | 2.8 ± 0.6 | 2.0 ± 0.2 | 1.4 ± 0.2 | ||
| At fourth peak | 3.6 ± 0.8 | 0.7 ± 0.3 | 0.6 ± 0.1 | 0.4 ± 0.1 | ||
| At fifth peak | 2.0 ± 0.2 | 0.2 ± 0.1 | 0 | 0 | ||
| Cell expansion | 4.7 ± 0.5 | 2.1 ± 0.2 | 2.0 ± 0.1 | 1.9 ± 0.1 | ||
| Population doubling | 2.3 ± 0.2 | 1.1 ± 0.2 | 1.0 ± 0.1 | 0.9 ± 0.1 | ||
. | CD8 T cells, day 7 . | . | CD4 T cells, day 17 . | . | ||
|---|---|---|---|---|---|---|
| Point of cell division . | Wild type . | c-myc+/- . | Wild type . | c-myc+/- . | ||
| Undivided | 31.4 ± 4.5 | 56.4 ± 2.4 | 45.5 ± 2.2 | 45.1 ± 2.2 | ||
| At first peak | 37.3 ± 2.2 | 29.2 ± 1.3 | 39.9 ± 0.1 | 42.7 ± 0.6 | ||
| At second peak | 17.7 ± 1.8 | 10.7 ± 2.3 | 11.4 ± 0.8 | 10.0 ± 1.0 | ||
| At third peak | 7.1 ± 0.6 | 2.8 ± 0.6 | 2.0 ± 0.2 | 1.4 ± 0.2 | ||
| At fourth peak | 3.6 ± 0.8 | 0.7 ± 0.3 | 0.6 ± 0.1 | 0.4 ± 0.1 | ||
| At fifth peak | 2.0 ± 0.2 | 0.2 ± 0.1 | 0 | 0 | ||
| Cell expansion | 4.7 ± 0.5 | 2.1 ± 0.2 | 2.0 ± 0.1 | 1.9 ± 0.1 | ||
| Population doubling | 2.3 ± 0.2 | 1.1 ± 0.2 | 1.0 ± 0.1 | 0.9 ± 0.1 | ||
Results are expressed as mean percentage ± SD of 2 to 3 individual mice. The percentage of CFSE-labeled donor wt and c-myc+/- CD8 and CD4 T cells present in each peak—7 and 17 days after adoptive transfer into wt recipients, respectively—are estimated according to Wells and colleagues.43