Primary antibodies
Antibody . | Source . | Species . | Clone or serum . | Isotype . | Dilution . |
|---|---|---|---|---|---|
| AID | E.K. (www.ascenion.de) | Rat | EK2-5G9 | G | 1:200 |
| BLIMP-1/PRDM1 | K. L. Calame, Columbia University | Mouse | 3H2E8 | G1 | 1:30 |
| MUM 1 | B. Falini, Perugia University | Mouse | 2H9 | G1 | 1:50 |
| MUM1/IRF4 | Santa Cruz Biotechnology (SCBT; Santa Cruz, CA) | Goat | sc-6059 | G | 1 μg/mL |
| c-Rel (NF-κB) | Oncogene Research (Cambridge, MA) | Rabbit | Ab-1 | G | 1:2000 |
| cMYC | SCBT | Rabbit | sc-764 | G | 0.5 μg/mL |
| Ki-67 | J. Gerdes, MIB Germany | Mouse | MIB 1 | G1 | 1:50 |
| Ki-67 | LabVision (Fremont, CA) | Rabbit | serum | G | 1:100 |
| CCNB1 (cyclin B1) | SCBT | Rabbit | sc-752 | G | 0.5 μg/mL |
| CDKN1B (p27) | BD PharMingen (San Diego, CA) | Mouse | 57 | G1 | 1 μg/mL |
| BCL6 | SCBT | Rabbit | sc-858 | G | 0.1 μg/mL |
| MTA3 | Calbiochem (San Diego, CA) | Rabbit | IM1012 | G | 0.5 μg/mL |
| PAX5 | SCBT | Goat | C-20 sc1974 | G | 1 μg/mL |
| PAX5 | BD PharMingen | Mouse | 24 | G1 | 1 μg/mL |
| IgD | Dako USA (Carpinteria, CA) | Rabbit | serum | G | 1:4000 |
| CD3e | Dako USA | Rabbit | serum | G | 0.5 μg/mL |
| CD20 | LabVision | Mouse | L26 | G2a | 0.5 μg/mL |
| CD23 | Novocastra (Newcastle-upon-Tyne, United Kingdom) | Mouse | 1B12 | G1 | 1:50 |
| CD30 | LabVision | Mouse | Ber-H2 | G1 | 1 μg/mL |
| CD138 | Serotec (Raleigh, NC) | Mouse | B-B4 | G1 | 1:200 |
| CD79a | LabVision | Rabbit | Sp18 | G | 1:200 |
| CCL22 (MDC) | PeproTech (Rocky Hill, NJ) | Rabbit | serum | G | 0.5 μg/mL |
| JunB | SCBT | Mouse | sc-8051 | G1 | 1 μg/mL |
| E47 | SCBT | Rabbit | sc-763 | G | 0.2 μg/mL |
| Id2 | Zymed/Invitrogen (South San Francisco, CA) | Rabbit | serum | G | 0.5 μg/mL |
| γH2AX | Upstate Biotechnology (Lake Placid, NY) | Mouse | JBW301 | G1 | 1 μg/mL |
| PhosphoH2B | Upstate Biotechnology | Rabbit | serum | G | 1:10 000 |
| TP53 | J. Bartek, Denmark | Mouse | BP53.11.1 | G2a | 1:100 |
| TP53 | J. Bartek, Denmark | Mouse | DO.1 | G2a | 1:100 |
| Negative control | Sigma (St Louis, MO) | Rabbit | serum | G | 1 μg/mL |
| Negative control | Sigma | Mouse | serum | G | 1 μg/mL |
Antibody . | Source . | Species . | Clone or serum . | Isotype . | Dilution . |
|---|---|---|---|---|---|
| AID | E.K. (www.ascenion.de) | Rat | EK2-5G9 | G | 1:200 |
| BLIMP-1/PRDM1 | K. L. Calame, Columbia University | Mouse | 3H2E8 | G1 | 1:30 |
| MUM 1 | B. Falini, Perugia University | Mouse | 2H9 | G1 | 1:50 |
| MUM1/IRF4 | Santa Cruz Biotechnology (SCBT; Santa Cruz, CA) | Goat | sc-6059 | G | 1 μg/mL |
| c-Rel (NF-κB) | Oncogene Research (Cambridge, MA) | Rabbit | Ab-1 | G | 1:2000 |
| cMYC | SCBT | Rabbit | sc-764 | G | 0.5 μg/mL |
| Ki-67 | J. Gerdes, MIB Germany | Mouse | MIB 1 | G1 | 1:50 |
| Ki-67 | LabVision (Fremont, CA) | Rabbit | serum | G | 1:100 |
| CCNB1 (cyclin B1) | SCBT | Rabbit | sc-752 | G | 0.5 μg/mL |
| CDKN1B (p27) | BD PharMingen (San Diego, CA) | Mouse | 57 | G1 | 1 μg/mL |
| BCL6 | SCBT | Rabbit | sc-858 | G | 0.1 μg/mL |
| MTA3 | Calbiochem (San Diego, CA) | Rabbit | IM1012 | G | 0.5 μg/mL |
| PAX5 | SCBT | Goat | C-20 sc1974 | G | 1 μg/mL |
| PAX5 | BD PharMingen | Mouse | 24 | G1 | 1 μg/mL |
| IgD | Dako USA (Carpinteria, CA) | Rabbit | serum | G | 1:4000 |
| CD3e | Dako USA | Rabbit | serum | G | 0.5 μg/mL |
| CD20 | LabVision | Mouse | L26 | G2a | 0.5 μg/mL |
| CD23 | Novocastra (Newcastle-upon-Tyne, United Kingdom) | Mouse | 1B12 | G1 | 1:50 |
| CD30 | LabVision | Mouse | Ber-H2 | G1 | 1 μg/mL |
| CD138 | Serotec (Raleigh, NC) | Mouse | B-B4 | G1 | 1:200 |
| CD79a | LabVision | Rabbit | Sp18 | G | 1:200 |
| CCL22 (MDC) | PeproTech (Rocky Hill, NJ) | Rabbit | serum | G | 0.5 μg/mL |
| JunB | SCBT | Mouse | sc-8051 | G1 | 1 μg/mL |
| E47 | SCBT | Rabbit | sc-763 | G | 0.2 μg/mL |
| Id2 | Zymed/Invitrogen (South San Francisco, CA) | Rabbit | serum | G | 0.5 μg/mL |
| γH2AX | Upstate Biotechnology (Lake Placid, NY) | Mouse | JBW301 | G1 | 1 μg/mL |
| PhosphoH2B | Upstate Biotechnology | Rabbit | serum | G | 1:10 000 |
| TP53 | J. Bartek, Denmark | Mouse | BP53.11.1 | G2a | 1:100 |
| TP53 | J. Bartek, Denmark | Mouse | DO.1 | G2a | 1:100 |
| Negative control | Sigma (St Louis, MO) | Rabbit | serum | G | 1 μg/mL |
| Negative control | Sigma | Mouse | serum | G | 1 μg/mL |