Table 1. Modulation of lipid metabolism... | American Society of Hematology

Table 1.

Modulation of lipid metabolism in ADP-stimulated human platelets

Treatment	TxB₂, ng/mL	12-S-HHTrE, ng/mL	12-R-HETE, ng/mL	Arachidonic acid, ng/mL
None	10 ± 1	14 ± 5	13 ± 2	72 ± 20
Aspirin	4 ± 1^*	7 ± 2^*	12 ± 3	105 ± 22^*
Dexamethasone
1 μM	9 ± 2	10 ± 3	7 ± 1^*	69 ± 15
3 μM	9 ± 1	9 ± 2	8 ± 2	66 ± 10
10 μM	7 ± 2	7 ± 2^*	9 ± 2	77 ± 16
Prednisolone
1 μM	6 ± 2^*	10 ± 2	7 ± 2^*	78 ± 12
3 μM	5 ± 1^*†	6 ± 3^*	8 ± 2	84 ± 15
10 μM	5 ± 2^*	5 ± 2^*	10 ± 2	69 ± 5
NCX-1015	4 ± 2^*†	6 ± 2^*	10 ± 2	55 ± 15

Treatment	TxB₂, ng/mL	12-S-HHTrE, ng/mL	12-R-HETE, ng/mL	Arachidonic acid, ng/mL
None	10 ± 1	14 ± 5	13 ± 2	72 ± 20
Aspirin	4 ± 1^*	7 ± 2^*	12 ± 3	105 ± 22^*
Dexamethasone
1 μM	9 ± 2	10 ± 3	7 ± 1^*	69 ± 15
3 μM	9 ± 1	9 ± 2	8 ± 2	66 ± 10
10 μM	7 ± 2	7 ± 2^*	9 ± 2	77 ± 16
Prednisolone
1 μM	6 ± 2^*	10 ± 2	7 ± 2^*	78 ± 12
3 μM	5 ± 1^*†	6 ± 3^*	8 ± 2	84 ± 15
10 μM	5 ± 2^*	5 ± 2^*	10 ± 2	69 ± 5
NCX-1015	4 ± 2^*†	6 ± 2^*	10 ± 2	55 ± 15

Lipid release was measured in human PRP by HPLC (“Materials and methods”). Basal values in unstimulated platelets were (ng/mL): 4 ± 1 for thromboxane B₂ (TxB₂), 6 ± 2 for 12-S-hydroxyheptadecatrienoic acid (12-S-HHTrE), 6 ± 1 for 12-R-hydroxyeicosatetraenoic acid (12-R-HETE), and 1 ± 1 for arachidonic acid (n = 6). Dexamethasone, prednisolone, aspirin, or NCX-1015 was added to PRP for 5 minutes prior to ADP (4 μM) addition and extracts were prepared 10 minutes later. Aspirin and NCX-1015 (nitro-prednisolone) were used at 10 μM. Data are mean ± SEM of 6 experiments performed with PRP collected from distinct donors.

P < .05 versus ADP alone

†

P < .05 versus corresponding Dex concentrations

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