For amplification of overlapping Triad1 RT-PCR fragments 2, 3, 4 (Figure 1) primer combinations T1V-T1AB, T1Z-T1P, and T1K-T1O were used, respectively. For 3′-RACE primers AO was used for cDNA synthesis followed by PCR using primers AP1 and T1D, followed by heminested PCR using primers AP1 and T1B. For 5′-RACE primers AP2 and T1X were used in PCR followed by heminested PCR using primers AP2 and T1Y. T1M4 and T1M5 were used to for the construction of a C-terminally myc tagged Triad1 PCR construct. The 30-bp myc-tag encoding sequence is underlined. Primers H158AF, H158AR, C161AF, and C161AR were used to construct Triad1 H158A and Triad1 C161, respectively. For generation of GST-Triad1 and GFP-Triad1 primer pairs T1exp1 and T1exp2 were used. For cloning full-length Triad1 in pCDNA3.1 and retroviral vectors primers T1M4 and T1exp2 were used. For cloning Triad^1-119 and Triad1^109-493 in pGAD vector primer combinations T1exp1-T1G1 and T1G2-T1exp2 were used, respectively. For Triad1 qPCR primers T1qF and T1qR and probe (TET-label) T1qP were used. BamHI and EcoRI sites introduced by PCR for cloning purposes are indicated (bold italics).