IL-8 secretion from PMNs adherent to TSP-4 variants
. | IL-8 secretion (pg/mL) . | . | |
|---|---|---|---|
| TSP-4 variant . | 4 h . | 24 h . | |
| PVP | 80 ± 14 | 150 ± 25 | |
| TSP-4 (A387) | 70 ± 10 | 200 ± 35 | |
| TSP-4 (P387) | 145 ± 12 | 500 ± 50 | |
| 3EGF–TSP-4 (A387) | 66 ± 8 | 190 ± 24 | |
| 3EGF–TSP-4 (P387) | 157 ± 12 | 485 ± 30 | |
. | IL-8 secretion (pg/mL) . | . | |
|---|---|---|---|
| TSP-4 variant . | 4 h . | 24 h . | |
| PVP | 80 ± 14 | 150 ± 25 | |
| TSP-4 (A387) | 70 ± 10 | 200 ± 35 | |
| TSP-4 (P387) | 145 ± 12 | 500 ± 50 | |
| 3EGF–TSP-4 (A387) | 66 ± 8 | 190 ± 24 | |
| 3EGF–TSP-4 (P387) | 157 ± 12 | 485 ± 30 | |
PMA-stimulated PMNs were allowed to adhere to the full-length or 3EGF–TSP variants (100 nM) for up to 24 hours. IL-8 levels were measured by ELISA in cell supernatants collected after 4 and 24 hours.