Assessment of competitive repopulating ability limiting dilution analysis
No. cells injected . | Wk 12 . | Wk 20 to 26 . |
|---|---|---|
| Hoxa-9-/- | ||
| 240 000 | 6/6 | 6/6 |
| 125 000 | 3/5 | 3/5 |
| 120 000 | 3/4 | 2/3 |
| 100 000 | 1/4 | 1/4 |
| 60 000 | 0/5 | 0/4 |
| 50 000 | 1/5 | 1/5 |
| 30 000 | 1/7 | 1/4 |
| 20 000 | 0/5 | 0/5 |
| 15 000 | ND | ND |
| 10 000 | 0/5 | ND |
| CRU frequency per 105 cells, ± 1 SE* (95% CI) | 0.61 (0.47-0.79) | 0.64 (0.49-0.84) |
| Wild type | ||
| 30 000 | 9/11 | 8/11 |
| 20 000 | ND | ND |
| 15 000 | 6/10 | 5/10 |
| 10 000 | 0/2 | 0/2 |
| 7 500 | 2/10 | 2/9 |
| 4 000 | 0/5 | ND |
| 3 750 | 0/4 | ND |
| CRU frequency per 105 cells, ± 1 SE* (95% CI) | 4.38 (3.42-5.60) | 4.00 (3.07-5.22) |
No. cells injected . | Wk 12 . | Wk 20 to 26 . |
|---|---|---|
| Hoxa-9-/- | ||
| 240 000 | 6/6 | 6/6 |
| 125 000 | 3/5 | 3/5 |
| 120 000 | 3/4 | 2/3 |
| 100 000 | 1/4 | 1/4 |
| 60 000 | 0/5 | 0/4 |
| 50 000 | 1/5 | 1/5 |
| 30 000 | 1/7 | 1/4 |
| 20 000 | 0/5 | 0/5 |
| 15 000 | ND | ND |
| 10 000 | 0/5 | ND |
| CRU frequency per 105 cells, ± 1 SE* (95% CI) | 0.61 (0.47-0.79) | 0.64 (0.49-0.84) |
| Wild type | ||
| 30 000 | 9/11 | 8/11 |
| 20 000 | ND | ND |
| 15 000 | 6/10 | 5/10 |
| 10 000 | 0/2 | 0/2 |
| 7 500 | 2/10 | 2/9 |
| 4 000 | 0/5 | ND |
| 3 750 | 0/4 | ND |
| CRU frequency per 105 cells, ± 1 SE* (95% CI) | 4.38 (3.42-5.60) | 4.00 (3.07-5.22) |
Results are expressed as number of mice repopulated with donor-derived cells (CD45.2+)/total mice in the cohort as measured by FACS analysis 12 to 20 weeks after transplantation. CRU frequency was calculated using limiting-dilution analysis (see “Materials and methods”). The results are a composite of 3 separate experiments involving 6 Hoxa-9-/- and 7 wild-type sibling control mice as donors.
ND indicates not determined; CI, confidence interval.
P = .001