Table 2.

ATP STD values in complex with p38γ inactive with CSH18

Proton1H, ppmSTD (%) ± errorSTD (%) ± errorSTD (%) ± error
CSH18 = 4 μMCSH18 = 20 μM
H8 8.51 1.07 ± 0.18 0.64 ± 0.18 2.02 ± 0.2 
H2 8.26 11.05 ± 0.36 9.77 ± 0.29 11.15 ± 0.25 
H1′ 6.144 3.4 ± 0.34 1.89 ± 0.33 4.48 ± 0.34 
H1′ 6.136 3.52 ± 0.33 2.28 ± 0.31 4.12 ± 0.32 
Proton1H, ppmSTD (%) ± errorSTD (%) ± errorSTD (%) ± error
CSH18 = 4 μMCSH18 = 20 μM
H8 8.51 1.07 ± 0.18 0.64 ± 0.18 2.02 ± 0.2 
H2 8.26 11.05 ± 0.36 9.77 ± 0.29 11.15 ± 0.25 
H1′ 6.144 3.4 ± 0.34 1.89 ± 0.33 4.48 ± 0.34 
H1′ 6.136 3.52 ± 0.33 2.28 ± 0.31 4.12 ± 0.32 

STD values in the interaction of p38γ with compound CSH18. The compound competes with ATP at the ATP binding site. A competition STD experiment was conducted, measuring the STD values of 50 μM ATP in the presence of 1 μM inactive p38γ under 3 conditions: with 4 μM of compound 18, with 20 μM of compound 18, and no compound 18 added.

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