Table 1.

Kinetic characterization of ADAMTS13 mutants

VariantKM (μM)kcat (min−1)kcat/KM (μM−1 × min−1)
WT 4.2 ± 1.3 217.8 ± 38.6 51.9 ± 18.5 
T4L 4.7 ± 1.8 193.7 ± 45.0 41.2 ± 18.5 
T8L 3.5 ± 1.8 190.2 ± 51.7 54.3 ± 31.6 
T4L/T8L 2.7 ± 0.8 206.2 ± 28.8 76.4 ± 25.1 
T4L/T8L(I380G) 3.9 ± 1.4 218.4 ± 42.5 56.0 ± 23.0 
VariantKM (μM)kcat (min−1)kcat/KM (μM−1 × min−1)
WT 4.2 ± 1.3 217.8 ± 38.6 51.9 ± 18.5 
T4L 4.7 ± 1.8 193.7 ± 45.0 41.2 ± 18.5 
T8L 3.5 ± 1.8 190.2 ± 51.7 54.3 ± 31.6 
T4L/T8L 2.7 ± 0.8 206.2 ± 28.8 76.4 ± 25.1 
T4L/T8L(I380G) 3.9 ± 1.4 218.4 ± 42.5 56.0 ± 23.0 

Values expressed as mean ± standard error.

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