Clinicobiological parameters of 48 patients with APL included in the study for validation of RAPID-CRISPR assay
| Sample ID . | PML::RARA isoform . | PML::RARA isoform by RAPID-CRISPR . | Age/sex . | Hemoglobin (g/dL) . | Risk group . | Abnormal promyelocytes (%) . |
|---|---|---|---|---|---|---|
| UPN1 | BCR3 | BCR3 | 23/F | 5.1 | High | 98 |
| UPN2 | BCR3 | BCR3 | 30/M | 9.6 | High | 90 |
| UPN3 | BCR3 | BCR3 | 37/M | 8.0 | High | 79 |
| UPN4 | BCR3 | BCR3 | 47/F | 5.9 | High | 98 |
| UPN5 | BCR3 | BCR3 | 32/M | 8.3 | Low | 32 |
| UPN6 | BCR3 | BCR3 | 32/M | 10.6 | Intermediate | 70 |
| UPN7 | BCR3 | BCR3 | 25/F | 5.2 | High | 90 |
| UPN8 | BCR3 | BCR3 | 53/M | 10.2 | Low | 32 |
| UPN9 | BCR3 | BCR3 | 28/M | 8.5 | High | 92 |
| UPN10 | BCR3 | BCR3 | 18/M | 5.3 | High | 80 |
| UPN11 | BCR3 | BCR3 | 45/M | 7.3 | Intermediate | 70 |
| UPN12 | BCR3 | BCR3 | 18/M | 5.9 | Intermediate | 15 |
| UPN13 | BCR3 | BCR3 | 41/M | 6.6 | Low | 44 |
| UPN14 | BCR3 | BCR3 | 35/M | 11.2 | High | 84 |
| UPN15 | BCR3 | BCR3 | 26/M | 5.9 | High | 91 |
| UPN16 | BCR1 | BCR1 | 44/M | 7.7 | Intermediate | 65 |
| UPN17 | BCR1 | BCR1 | 22/M | 8.0 | Intermediate | 40 |
| UPN18 | BCR1 | BCR1 | 17/M | 9.7 | Low | 90 |
| UPN19 | BCR1 | BCR1 | 31/F | 5.2 | Intermediate | 78 |
| UPN20 | BCR1 | BCR1 | 19/F | 8.6 | Low | 85 |
| UPN21 | BCR1 | BCR1 | 55/F | 10.7 | Low | 87 |
| UPN22 | BCR1 | BCR1 | 33/M | 8.1 | Intermediate | 59 |
| UPN23 | BCR1 | BCR1 | 45/M | 8.9 | Intermediate | 20 |
| UPN24 | BCR1 | BCR1 | 27/F | 6.8 | Intermediate | 90 |
| UPN25 | BCR3 | BCR3 | 12/M | 8.5 | Low | 79 |
| UPN26 | BCR1 | BCR1 | 14/F | 6.5 | High | 86 |
| UPN27 | BCR1 | BCR1 | 21/M | 7.5 | High | 84 |
| UPN28 | BCR1 | BCR1 | 13/F | 9.7 | Intermediate | 70 |
| UPN29 | BCR1 | BCR1 | 56/F | 9.4 | Intermediate | 85 |
| UPN30 | BCR1 | BCR1 | 26/M | 8.4 | Intermediate | 70 |
| UPN31 | BCR1 | BCR1 | 15/M | 8.4 | Low | 90 |
| UPN32 | BCR1 | BCR1 | 28/F | 8.6 | Intermediate | 94 |
| UPN33 | BCR1 | BCR1 | 51/M | 8.7 | Low | 75 |
| UPN34 | BCR1 | BCR1 | 47/F | 8.7 | Low | 43 |
| UPN35 | BCR1 | BCR1 | 6/F | 12.2 | Low | 35 |
| UPN36 | BCR1 | BCR1 | 37/M | 7.1 | Intermediate | 91 |
| UPN37 | BCR1 | BCR1 | 22/M | 6.7 | Low | 55 |
| UPN38 | BCR1 | BCR1 | 9/M | 6.5 | Low | 91 |
| UPN39 | BCR1 | BCR1 | 51/F | 9.5 | Intermediate | 86 |
| UPN40 | BCR3 | BCR3 | 20/M | 9.1 | Intermediate | 80 |
| UPN41 | BCR3 | BCR3 | 36/F | 9.5 | High | 77 |
| UPN42 | BCR3 | BCR3 | 20/F | 7.3 | High | 91 |
| UPN43 | BCR3 | BCR3 | 20/M | 9.3 | High | 84 |
| UPN44 | BCR3 | BCR3 | 23/F | 5.1 | High | 98 |
| UPN45 | BCR3 | BCR3 | 9/M | 9.0 | Intermediate | 70 |
| UPN46 | BCR2 | BCR2 | 24/F | 9.6 | High | 90 |
| UPN47 | BCR2 | BCR2 | 48/F | 10.1 | Low | 83 |
| UPN48 | BCR2 | BCR2 | 41/M | 9.1 | High | 85 |
| UPN49 | BCR1 | BCR1 | 43/M | 7.4 | Intermediate | 69 |
| UPN50 | BCR1 | BCR1 | 11/M | 7.7 | High | 37 |
| UPN51 | BCR1 | BCR1 | 47/F | 7.3 | Intermediate | 85 |
| UPN52 | BCR3 | BCR3 | 46/F | 8.6 | Intermediate | 62 |
| UPN53 | BCR3 | BCR3 | 32/F | 6.4 | High | 95 |
| UPN54 | BCR1 | BCR1 | 11/M | 6.7 | High | 85 |
| UPN55 | BCR3 | BCR3 | 7/F | 9.4 | Low | 80 |
| UPN56 | BCR3 | BCR3 | 67/M | 9.9 | High | 93 |
| UPN57 | BCR3 | BCR3 | 9/M | 9 | Intermediate | 34 |
| UPN58 | BCR3 | BCR3 | 40/M | 9.4 | High | 68 |
| UPN59 | BCR1 | BCR1 | 16/M | 9.3 | Intermediate | 34 |
| UPN60 | BCR1 | BCR1 | 25/M | 6.5 | Low | 83 |
| UPN61 | BCR1 | BCR1 | 38/M | 8.9 | High | 71 |
| UPN62 | BCR3 | BCR3 | 32/M | 8.1 | Intermediate | 86 |
| UPN63 | BCR1 | BCR1 | 11/M | 6.7 | High | 85 |
| UPN64 | BCR3 | BCR3 | 37/M | 9.5 | Intermediate | 77 |
| UPN65 | BCR3 | BCR3 | 43/F | 8.7 | High | 85 |
| UPN66 | BCR1 | BCR1 | 25/M | 6.5 | Low | 83 |
| Sample ID . | PML::RARA isoform . | PML::RARA isoform by RAPID-CRISPR . | Age/sex . | Hemoglobin (g/dL) . | Risk group . | Abnormal promyelocytes (%) . |
|---|---|---|---|---|---|---|
| UPN1 | BCR3 | BCR3 | 23/F | 5.1 | High | 98 |
| UPN2 | BCR3 | BCR3 | 30/M | 9.6 | High | 90 |
| UPN3 | BCR3 | BCR3 | 37/M | 8.0 | High | 79 |
| UPN4 | BCR3 | BCR3 | 47/F | 5.9 | High | 98 |
| UPN5 | BCR3 | BCR3 | 32/M | 8.3 | Low | 32 |
| UPN6 | BCR3 | BCR3 | 32/M | 10.6 | Intermediate | 70 |
| UPN7 | BCR3 | BCR3 | 25/F | 5.2 | High | 90 |
| UPN8 | BCR3 | BCR3 | 53/M | 10.2 | Low | 32 |
| UPN9 | BCR3 | BCR3 | 28/M | 8.5 | High | 92 |
| UPN10 | BCR3 | BCR3 | 18/M | 5.3 | High | 80 |
| UPN11 | BCR3 | BCR3 | 45/M | 7.3 | Intermediate | 70 |
| UPN12 | BCR3 | BCR3 | 18/M | 5.9 | Intermediate | 15 |
| UPN13 | BCR3 | BCR3 | 41/M | 6.6 | Low | 44 |
| UPN14 | BCR3 | BCR3 | 35/M | 11.2 | High | 84 |
| UPN15 | BCR3 | BCR3 | 26/M | 5.9 | High | 91 |
| UPN16 | BCR1 | BCR1 | 44/M | 7.7 | Intermediate | 65 |
| UPN17 | BCR1 | BCR1 | 22/M | 8.0 | Intermediate | 40 |
| UPN18 | BCR1 | BCR1 | 17/M | 9.7 | Low | 90 |
| UPN19 | BCR1 | BCR1 | 31/F | 5.2 | Intermediate | 78 |
| UPN20 | BCR1 | BCR1 | 19/F | 8.6 | Low | 85 |
| UPN21 | BCR1 | BCR1 | 55/F | 10.7 | Low | 87 |
| UPN22 | BCR1 | BCR1 | 33/M | 8.1 | Intermediate | 59 |
| UPN23 | BCR1 | BCR1 | 45/M | 8.9 | Intermediate | 20 |
| UPN24 | BCR1 | BCR1 | 27/F | 6.8 | Intermediate | 90 |
| UPN25 | BCR3 | BCR3 | 12/M | 8.5 | Low | 79 |
| UPN26 | BCR1 | BCR1 | 14/F | 6.5 | High | 86 |
| UPN27 | BCR1 | BCR1 | 21/M | 7.5 | High | 84 |
| UPN28 | BCR1 | BCR1 | 13/F | 9.7 | Intermediate | 70 |
| UPN29 | BCR1 | BCR1 | 56/F | 9.4 | Intermediate | 85 |
| UPN30 | BCR1 | BCR1 | 26/M | 8.4 | Intermediate | 70 |
| UPN31 | BCR1 | BCR1 | 15/M | 8.4 | Low | 90 |
| UPN32 | BCR1 | BCR1 | 28/F | 8.6 | Intermediate | 94 |
| UPN33 | BCR1 | BCR1 | 51/M | 8.7 | Low | 75 |
| UPN34 | BCR1 | BCR1 | 47/F | 8.7 | Low | 43 |
| UPN35 | BCR1 | BCR1 | 6/F | 12.2 | Low | 35 |
| UPN36 | BCR1 | BCR1 | 37/M | 7.1 | Intermediate | 91 |
| UPN37 | BCR1 | BCR1 | 22/M | 6.7 | Low | 55 |
| UPN38 | BCR1 | BCR1 | 9/M | 6.5 | Low | 91 |
| UPN39 | BCR1 | BCR1 | 51/F | 9.5 | Intermediate | 86 |
| UPN40 | BCR3 | BCR3 | 20/M | 9.1 | Intermediate | 80 |
| UPN41 | BCR3 | BCR3 | 36/F | 9.5 | High | 77 |
| UPN42 | BCR3 | BCR3 | 20/F | 7.3 | High | 91 |
| UPN43 | BCR3 | BCR3 | 20/M | 9.3 | High | 84 |
| UPN44 | BCR3 | BCR3 | 23/F | 5.1 | High | 98 |
| UPN45 | BCR3 | BCR3 | 9/M | 9.0 | Intermediate | 70 |
| UPN46 | BCR2 | BCR2 | 24/F | 9.6 | High | 90 |
| UPN47 | BCR2 | BCR2 | 48/F | 10.1 | Low | 83 |
| UPN48 | BCR2 | BCR2 | 41/M | 9.1 | High | 85 |
| UPN49 | BCR1 | BCR1 | 43/M | 7.4 | Intermediate | 69 |
| UPN50 | BCR1 | BCR1 | 11/M | 7.7 | High | 37 |
| UPN51 | BCR1 | BCR1 | 47/F | 7.3 | Intermediate | 85 |
| UPN52 | BCR3 | BCR3 | 46/F | 8.6 | Intermediate | 62 |
| UPN53 | BCR3 | BCR3 | 32/F | 6.4 | High | 95 |
| UPN54 | BCR1 | BCR1 | 11/M | 6.7 | High | 85 |
| UPN55 | BCR3 | BCR3 | 7/F | 9.4 | Low | 80 |
| UPN56 | BCR3 | BCR3 | 67/M | 9.9 | High | 93 |
| UPN57 | BCR3 | BCR3 | 9/M | 9 | Intermediate | 34 |
| UPN58 | BCR3 | BCR3 | 40/M | 9.4 | High | 68 |
| UPN59 | BCR1 | BCR1 | 16/M | 9.3 | Intermediate | 34 |
| UPN60 | BCR1 | BCR1 | 25/M | 6.5 | Low | 83 |
| UPN61 | BCR1 | BCR1 | 38/M | 8.9 | High | 71 |
| UPN62 | BCR3 | BCR3 | 32/M | 8.1 | Intermediate | 86 |
| UPN63 | BCR1 | BCR1 | 11/M | 6.7 | High | 85 |
| UPN64 | BCR3 | BCR3 | 37/M | 9.5 | Intermediate | 77 |
| UPN65 | BCR3 | BCR3 | 43/F | 8.7 | High | 85 |
| UPN66 | BCR1 | BCR1 | 25/M | 6.5 | Low | 83 |
F, female; M, male.