Whole-blood leukocyte prevalence
| . | Allele . | Young WT (n = 9F) . | Old WT (n = 9F) . | Old TNF–/– (n = 7F) . | |||
|---|---|---|---|---|---|---|---|
| Mean (SD) . | Median (IQR) . | Mean (SD) . | Median (IQR) . | Mean (SD) . | Median (IQR) . | ||
| % CD45+leukocytes | |||||||
| Neutrophils | WT (CD45.1) | 5.28 (2.84) | 5.2 (3.07-6.24) | 15.13 (10.6) | 11.7 (7.035-24.25) | 7.38 (3.45) | 6.46 (4.83-8.94) |
| Tet2–/– (CD45.2) | 13.44 (3.23) | 12.9 (11.1-15.2) | 20.13 (5.88) | 17.8 (15.75-24.35) | 14.54 (2.21) | 14.7 (13.1-16.5) | |
| Monocytes | WT (CD45.1) | 6.27 (2.01) | 2.018 (6.27-0.672) | 15.15 (8.07) | 8.07 (15.15-2.69) | 7.54 (2.99) | 2.99 (7.54-1.13) |
| Tet2–/– (CD45.2) | 13.29 (4.01) | 4.016 (13.29-1.33) | 19.82 (18.12) | 18.12 (19.82-6.04) | 10.2 (1.93) | 1.93 (10.2-0.73) | |
| Ly6Chigh monocytes | WT (CD45.1) | 1.58 (0.79) | 1.42 (0.76-2.54) | 4.791 (4.15) | 3.76 (2.53-4.68) | 2.24 (0.86) | 1.97 (1.66-3.11) |
| Tet2–/– (CD45.2) | 4.19 (1.80) | 3.6 (2.92-5.175) | 6.75 (2.07) | 5.75 (5.32-8.63) | 4.21 (1.08) | 3.68 (3.41-4.96) | |
| B cells | WT (CD45.1) | 59.98 (8.96) | 61.6 (52.25-67.55) | 42.57 (20.34) | 51.2 (24.4-57.45) | 63.13 (20.55) | 70.3 (35.9-78.8) |
| Tet2–/– (CD45.2) | 52.6 (8.44) | 54.4 (46.5-59.7) | 47.12 (17.34) | 48.1 (45.95-59.05) | 65.84 (7.53) | 69.8 (57.5-70.9) | |
| T cells | WT (CD45.1) | 22.97 (8.52) | 23.3 (16.85-30.05) | 11.57 (4.35) | 11.65 (8.44-16) | 11.24 (4.19) | 10.5 (9.13-15.4) |
| Tet2–/– (CD45.2) | 14.21 (2.57) | 13.7 (11.7-16) | 4.028 (1.38) | 4.43 (2.96-5.19) | 5.14 (1.211) | 4.84 (4.32-6.71) | |
| CD8+ T cells | WT (CD45.1) | 8.66 (3.36) | 9.13 (5.08-12.1) | 3.89 (1.45) | 4.17 (2.70-4.82) | 3.28 (1.301) | 3.46 (2.51-4.54) |
| Tet2–/– (CD45.2) | 4.12 (0.73) | 4.29 (3.35-4.63) | 1.13 (0.42) | 1.24 (0.79-1.36) | 1.28 (0.36) | 1.14 (1.02-1.7) | |
| CD4+ T cells | WT (CD45.1) | 13.34 (5.60) | 12 (10.55-17.45) | 5.98 (2.54) | 5.97 (4.30-8.22) | 7.43 (2.787) | 6.78 (5.73-10) |
| Tet2–/– (CD45.2) | 9.63 (1.874) | 8.71 (8.17-11) | 2.70 (0.97) | 2.80 (2.00-3.64) | 3.69 (0.839) | 3.48 (3.13-4.85) | |
| % CD8+T cells | |||||||
| Naïve CD8+ | WT (CD45.1) | 36.46 (9.20) | 36.9 (29.9-42.05) | 16.19 (4.58) | 17.6 (14.35-19.7) | 30.34 (3.37) | 29.1 (29-33.1) |
| Tet2–/– (CD45.2) | 39.28 (5.80) | 37.1 (33.8-45.3) | 13.84 (3.89) | 13.45 (10.4-17.38) | 24.97 (6.36) | 26 (16.9-29.8) | |
| CD8+ TCM | WT (CD45.1) | 52.84 (5.73) | 53.4 (49.35-57.55) | 69.55 (7.42) | 66.75 (64.25-78.23) | 59.66 (3.35) | 60.4 (56.6-62.8) |
| Tet2–/– (CD45.2) | 52.1 (4.59) | 49.6 (48.1-56.6) | 63.9 (7.81) | 63.7 (56.73-70.45) | 49.57 (8.16) | 48.7 (43-55.9) | |
| CD8+ TEM | WT (CD45.1) | 9.911 (10.68) | 5.18 (3.97-13.65) | 13.8 (9.61) | 13.75 (5.695-17.18) | 8.836 (2.10) | 8.13 (7.64-9.95) |
| Tet2–/– (CD45.2) | 8.163 (4.08) | 7.12 (5.06-11) | 21.95 (7.43) | 20 (15.35-28.08) | 24.33 (11.6) | 21 (14-39.5) | |
| CD8+ TVM | WT (CD45.1) | 47.22 (5.81) | 48.2 (42.25-52.1) | 53.69 (7.24) | 54.95 (46.43-60.55) | 49.19 (5.55) | 48.7 (44.2-55.1) |
| Tet2–/– (CD45.2) | 46.34 (4.24) | 45.7 (42.4-50.15) | 50.96 (9.70) | 49.35 (43.68-59.7) | 42.74 (7.54) | 44.9 (38.4-50.1) | |
| % CD4+T cells | |||||||
| Naïve CD4+ | WT (CD45.1) | 30.64 (5.64) | 31.7 (25.25-34.6) | 17.58 (3.44) | 18.45 (15.9-19.3) | 21.49 (3.36) | 19.8 (18.9-24) |
| Tet2–/– (CD45.2) | 23.58 (1.69) | 23.7 (23.05-24.35) | 17.24 (2.56) | 18.05 (14.68-19.13) | 17.59 (2.46) | 16.7 (15.4-20.5) | |
| CD4+ TCM | WT (CD45.1) | 56.97 (4.83) | 56.4 (53.05-62.65) | 54.23 (7.29) | 55.2 (46.95-59.7) | 47.49 (8.97) | 48.5 (38-55.7) |
| Tet2–/– (CD45.2) | 62.51 (3.81) | 61.7 (59.55-65.85) | 51.7 (6.15) | 51.65 (45.05-58.23) | 47.36 (7.36) | 49.7 (41.5-52.9) | |
| CD4+ TEM | WT (CD45.1) | 11.51 (3.84) | 13.1 (8.58-14.05) | 27.45 (8.04) | 25.1 (20.48-35.45) | 27.89 (9.64) | 27.3 (18.1-34.7) |
| Tet2–/– (CD45.2) | 13.17 (3.96) | 13.9 (9.09-17.1) | 30.19 (5.99) | 33.1 (23.7-34.8) | 33.06 (7.45) | 30 (28-38.5) | |
| CD4+ TVM | WT (CD45.1) | 27.2 (4.00) | 28.6 (23.7-30.25) | 49.69 (5.58) | 49.9 (45.65-54.53) | 33.94 (5.53) | 30.8 (30.1-39.6) |
| Tet2–/– (CD45.2) | 24.28 (3.70) | 26.3 (21.2-27.05) | 37.11 (4.37) | 36.75 (34.03-40.4) | 27.89 (4.66) | 25.8 (24.6-31.4) | |
| . | Allele . | Young WT (n = 9F) . | Old WT (n = 9F) . | Old TNF–/– (n = 7F) . | |||
|---|---|---|---|---|---|---|---|
| Mean (SD) . | Median (IQR) . | Mean (SD) . | Median (IQR) . | Mean (SD) . | Median (IQR) . | ||
| % CD45+leukocytes | |||||||
| Neutrophils | WT (CD45.1) | 5.28 (2.84) | 5.2 (3.07-6.24) | 15.13 (10.6) | 11.7 (7.035-24.25) | 7.38 (3.45) | 6.46 (4.83-8.94) |
| Tet2–/– (CD45.2) | 13.44 (3.23) | 12.9 (11.1-15.2) | 20.13 (5.88) | 17.8 (15.75-24.35) | 14.54 (2.21) | 14.7 (13.1-16.5) | |
| Monocytes | WT (CD45.1) | 6.27 (2.01) | 2.018 (6.27-0.672) | 15.15 (8.07) | 8.07 (15.15-2.69) | 7.54 (2.99) | 2.99 (7.54-1.13) |
| Tet2–/– (CD45.2) | 13.29 (4.01) | 4.016 (13.29-1.33) | 19.82 (18.12) | 18.12 (19.82-6.04) | 10.2 (1.93) | 1.93 (10.2-0.73) | |
| Ly6Chigh monocytes | WT (CD45.1) | 1.58 (0.79) | 1.42 (0.76-2.54) | 4.791 (4.15) | 3.76 (2.53-4.68) | 2.24 (0.86) | 1.97 (1.66-3.11) |
| Tet2–/– (CD45.2) | 4.19 (1.80) | 3.6 (2.92-5.175) | 6.75 (2.07) | 5.75 (5.32-8.63) | 4.21 (1.08) | 3.68 (3.41-4.96) | |
| B cells | WT (CD45.1) | 59.98 (8.96) | 61.6 (52.25-67.55) | 42.57 (20.34) | 51.2 (24.4-57.45) | 63.13 (20.55) | 70.3 (35.9-78.8) |
| Tet2–/– (CD45.2) | 52.6 (8.44) | 54.4 (46.5-59.7) | 47.12 (17.34) | 48.1 (45.95-59.05) | 65.84 (7.53) | 69.8 (57.5-70.9) | |
| T cells | WT (CD45.1) | 22.97 (8.52) | 23.3 (16.85-30.05) | 11.57 (4.35) | 11.65 (8.44-16) | 11.24 (4.19) | 10.5 (9.13-15.4) |
| Tet2–/– (CD45.2) | 14.21 (2.57) | 13.7 (11.7-16) | 4.028 (1.38) | 4.43 (2.96-5.19) | 5.14 (1.211) | 4.84 (4.32-6.71) | |
| CD8+ T cells | WT (CD45.1) | 8.66 (3.36) | 9.13 (5.08-12.1) | 3.89 (1.45) | 4.17 (2.70-4.82) | 3.28 (1.301) | 3.46 (2.51-4.54) |
| Tet2–/– (CD45.2) | 4.12 (0.73) | 4.29 (3.35-4.63) | 1.13 (0.42) | 1.24 (0.79-1.36) | 1.28 (0.36) | 1.14 (1.02-1.7) | |
| CD4+ T cells | WT (CD45.1) | 13.34 (5.60) | 12 (10.55-17.45) | 5.98 (2.54) | 5.97 (4.30-8.22) | 7.43 (2.787) | 6.78 (5.73-10) |
| Tet2–/– (CD45.2) | 9.63 (1.874) | 8.71 (8.17-11) | 2.70 (0.97) | 2.80 (2.00-3.64) | 3.69 (0.839) | 3.48 (3.13-4.85) | |
| % CD8+T cells | |||||||
| Naïve CD8+ | WT (CD45.1) | 36.46 (9.20) | 36.9 (29.9-42.05) | 16.19 (4.58) | 17.6 (14.35-19.7) | 30.34 (3.37) | 29.1 (29-33.1) |
| Tet2–/– (CD45.2) | 39.28 (5.80) | 37.1 (33.8-45.3) | 13.84 (3.89) | 13.45 (10.4-17.38) | 24.97 (6.36) | 26 (16.9-29.8) | |
| CD8+ TCM | WT (CD45.1) | 52.84 (5.73) | 53.4 (49.35-57.55) | 69.55 (7.42) | 66.75 (64.25-78.23) | 59.66 (3.35) | 60.4 (56.6-62.8) |
| Tet2–/– (CD45.2) | 52.1 (4.59) | 49.6 (48.1-56.6) | 63.9 (7.81) | 63.7 (56.73-70.45) | 49.57 (8.16) | 48.7 (43-55.9) | |
| CD8+ TEM | WT (CD45.1) | 9.911 (10.68) | 5.18 (3.97-13.65) | 13.8 (9.61) | 13.75 (5.695-17.18) | 8.836 (2.10) | 8.13 (7.64-9.95) |
| Tet2–/– (CD45.2) | 8.163 (4.08) | 7.12 (5.06-11) | 21.95 (7.43) | 20 (15.35-28.08) | 24.33 (11.6) | 21 (14-39.5) | |
| CD8+ TVM | WT (CD45.1) | 47.22 (5.81) | 48.2 (42.25-52.1) | 53.69 (7.24) | 54.95 (46.43-60.55) | 49.19 (5.55) | 48.7 (44.2-55.1) |
| Tet2–/– (CD45.2) | 46.34 (4.24) | 45.7 (42.4-50.15) | 50.96 (9.70) | 49.35 (43.68-59.7) | 42.74 (7.54) | 44.9 (38.4-50.1) | |
| % CD4+T cells | |||||||
| Naïve CD4+ | WT (CD45.1) | 30.64 (5.64) | 31.7 (25.25-34.6) | 17.58 (3.44) | 18.45 (15.9-19.3) | 21.49 (3.36) | 19.8 (18.9-24) |
| Tet2–/– (CD45.2) | 23.58 (1.69) | 23.7 (23.05-24.35) | 17.24 (2.56) | 18.05 (14.68-19.13) | 17.59 (2.46) | 16.7 (15.4-20.5) | |
| CD4+ TCM | WT (CD45.1) | 56.97 (4.83) | 56.4 (53.05-62.65) | 54.23 (7.29) | 55.2 (46.95-59.7) | 47.49 (8.97) | 48.5 (38-55.7) |
| Tet2–/– (CD45.2) | 62.51 (3.81) | 61.7 (59.55-65.85) | 51.7 (6.15) | 51.65 (45.05-58.23) | 47.36 (7.36) | 49.7 (41.5-52.9) | |
| CD4+ TEM | WT (CD45.1) | 11.51 (3.84) | 13.1 (8.58-14.05) | 27.45 (8.04) | 25.1 (20.48-35.45) | 27.89 (9.64) | 27.3 (18.1-34.7) |
| Tet2–/– (CD45.2) | 13.17 (3.96) | 13.9 (9.09-17.1) | 30.19 (5.99) | 33.1 (23.7-34.8) | 33.06 (7.45) | 30 (28-38.5) | |
| CD4+ TVM | WT (CD45.1) | 27.2 (4.00) | 28.6 (23.7-30.25) | 49.69 (5.58) | 49.9 (45.65-54.53) | 33.94 (5.53) | 30.8 (30.1-39.6) |
| Tet2–/– (CD45.2) | 24.28 (3.70) | 26.3 (21.2-27.05) | 37.11 (4.37) | 36.75 (34.03-40.4) | 27.89 (4.66) | 25.8 (24.6-31.4) | |
Summary statistics of peripheral blood leukocyte proportions in young WT, old WT, and old TNF–/– recipient mice after engraftment of WT (CD45.1+) and Tet2–/– (CD45.2+) cells.
Abbreviations are explained in Table 1.