Whole-blood leukocyte cell numbers by group and allele type
| Recipient . | Allele . | Young WT (n = 9F) . | Old WT (n = 9F) . | Old TNF–/– (n = 7F) . | |||
|---|---|---|---|---|---|---|---|
| Mean (SD) . | Median (IQR) . | Mean (SD) . | Median (IQR) . | Mean (SD) . | Median (IQR) . | ||
| Myeloid | |||||||
| Neutrophils | WT (CD45.1) | 11.3 (5.32) | 9.129 (6.78-15.58) | 38.16 (35.96) | 25.46 (15.08-51.17) | 28.29 (21.01) | 19.75 (17.69-43.87) |
| Tet2–/– (CD45.2) | 164.9 (64.24) | 164.1 (112-215.5) | 400.3 (299.4) | 305.7 (261.1-426.2) | 316.6 (108) | 332.8 (228.3-380.6) | |
| Monocytes | WT (CD45.1) | 15.68 (12.51) | 12.74 (8.048-18.16) | 34.95 (14.51) | 39.66 (19.17-45.71) | 27.71 (18.35) | 22.17 (16.6-35.3) |
| Tet2–/– (CD45.2) | 170.8 (91.24) | 150.3 (103.6-248.7) | 260.4 (120.6) | 193 (184.2-402.9) | 218.7 (68.06) | 224.3 (147.2-278.2) | |
| Ly6Chigh monocytes | WT (CD45.1) | 3.90 (3.50) | 3.038 (1.66-4.765) | 10.04 (5.69) | 11.03 (4.57-13.06) | 8.118 (5.10) | 6.862 (5.48-11.65) |
| Tet2–/– (CD45.2) | 52.44 (28.99) | 43.93 (27.43-80.43) | 118 (54.79) | 95.63 (75.98-179.2) | 89.26 (27.75) | 91.14 (60.79-117.3) | |
| Lymphoid | |||||||
| B cells | WT (CD45.1) | 156.5 (94.43) | 139.9 (92.75-185.6) | 108.6 (31.94) | 104.4 (82.2-136.1) | 176.5 (63.27) | 197.2 (118.9-222.7) |
| Tet2–/– (CD45.2) | 770.6 (434.8) | 626 (461.8-999.2) | 867.6 (328.2) | 833.6 (593.7-971) | 1109 (305.7) | 1154 (749.8-1304) | |
| T cells | WT (CD45.1) | 56.72 (36.5) | 46.57 (28.31-83.66) | 23.54 (8.336) | 25.3 (14.82-30.59) | 34.64 (25.39) | 33.09 (9.829-52.03) |
| Tet2–/– (CD45.2) | 183.8 (66.22) | 194.3 (137.5-213.8) | 62.78 (32.63) | 58.05 (40.19-84.66) | 88.38 (46.48) | 75.93 (50.38-103.7) | |
| CD8+ T cells | WT (CD45.1) | 21.35 (14.37) | 18.09 (10.44-28.78) | 7.969 (3.05) | 7.943 (5.092-10.67) | 10.28 (7.954) | 9.916 (2.583-14.87) |
| Tet2–/– (CD45.2) | 53.68 (20.76) | 52.32 (40.32-63.94) | 17.85 (10.88) | 17.6 (10.13-21.05) | 22.34 (13.16) | 18.31 (11.9-26.32) | |
| CD4+ T cells | WT (CD45.1) | 33.08 (22.01) | 27.7 (14.6-51.96) | 12.36 (5.414) | 13.78 (6.318-17.17) | 22.93 (16.63) | 21.41 (7.067-35.83) |
| Tet2–/– (CD45.2) | 124.1 (43.91) | 128.9 (89.41-146) | 42.1 (21.35) | 39.86 (25.33-59.83) | 63.15 (31.8) | 56.18 (36.86-73.87) | |
| Recipient . | Allele . | Young WT (n = 9F) . | Old WT (n = 9F) . | Old TNF–/– (n = 7F) . | |||
|---|---|---|---|---|---|---|---|
| Mean (SD) . | Median (IQR) . | Mean (SD) . | Median (IQR) . | Mean (SD) . | Median (IQR) . | ||
| Myeloid | |||||||
| Neutrophils | WT (CD45.1) | 11.3 (5.32) | 9.129 (6.78-15.58) | 38.16 (35.96) | 25.46 (15.08-51.17) | 28.29 (21.01) | 19.75 (17.69-43.87) |
| Tet2–/– (CD45.2) | 164.9 (64.24) | 164.1 (112-215.5) | 400.3 (299.4) | 305.7 (261.1-426.2) | 316.6 (108) | 332.8 (228.3-380.6) | |
| Monocytes | WT (CD45.1) | 15.68 (12.51) | 12.74 (8.048-18.16) | 34.95 (14.51) | 39.66 (19.17-45.71) | 27.71 (18.35) | 22.17 (16.6-35.3) |
| Tet2–/– (CD45.2) | 170.8 (91.24) | 150.3 (103.6-248.7) | 260.4 (120.6) | 193 (184.2-402.9) | 218.7 (68.06) | 224.3 (147.2-278.2) | |
| Ly6Chigh monocytes | WT (CD45.1) | 3.90 (3.50) | 3.038 (1.66-4.765) | 10.04 (5.69) | 11.03 (4.57-13.06) | 8.118 (5.10) | 6.862 (5.48-11.65) |
| Tet2–/– (CD45.2) | 52.44 (28.99) | 43.93 (27.43-80.43) | 118 (54.79) | 95.63 (75.98-179.2) | 89.26 (27.75) | 91.14 (60.79-117.3) | |
| Lymphoid | |||||||
| B cells | WT (CD45.1) | 156.5 (94.43) | 139.9 (92.75-185.6) | 108.6 (31.94) | 104.4 (82.2-136.1) | 176.5 (63.27) | 197.2 (118.9-222.7) |
| Tet2–/– (CD45.2) | 770.6 (434.8) | 626 (461.8-999.2) | 867.6 (328.2) | 833.6 (593.7-971) | 1109 (305.7) | 1154 (749.8-1304) | |
| T cells | WT (CD45.1) | 56.72 (36.5) | 46.57 (28.31-83.66) | 23.54 (8.336) | 25.3 (14.82-30.59) | 34.64 (25.39) | 33.09 (9.829-52.03) |
| Tet2–/– (CD45.2) | 183.8 (66.22) | 194.3 (137.5-213.8) | 62.78 (32.63) | 58.05 (40.19-84.66) | 88.38 (46.48) | 75.93 (50.38-103.7) | |
| CD8+ T cells | WT (CD45.1) | 21.35 (14.37) | 18.09 (10.44-28.78) | 7.969 (3.05) | 7.943 (5.092-10.67) | 10.28 (7.954) | 9.916 (2.583-14.87) |
| Tet2–/– (CD45.2) | 53.68 (20.76) | 52.32 (40.32-63.94) | 17.85 (10.88) | 17.6 (10.13-21.05) | 22.34 (13.16) | 18.31 (11.9-26.32) | |
| CD4+ T cells | WT (CD45.1) | 33.08 (22.01) | 27.7 (14.6-51.96) | 12.36 (5.414) | 13.78 (6.318-17.17) | 22.93 (16.63) | 21.41 (7.067-35.83) |
| Tet2–/– (CD45.2) | 124.1 (43.91) | 128.9 (89.41-146) | 42.1 (21.35) | 39.86 (25.33-59.83) | 63.15 (31.8) | 56.18 (36.86-73.87) | |
All cell numbers are cells per μL. Summary statistics of peripheral blood leukocyte cell numbers in young WT, old WT, and old TNF–/– recipient mice after engraftment of WT (CD45.1+) and Tet2–/– (CD45.2+) cells.
F, female; IQR, interquartile range; SD, standard deviation.