Blood counts, platelet surface parameters, and recorded pH at day 0 and after 7 days of storage
| . | Day 0 (PC) . | Day 0 (isolated) . | Day 7 (PC) . | Day 7 (isolated) . |
|---|---|---|---|---|
| Platelet count # (× 109/L) | 1507.6 ± 373 | 1516 ± 284 | 1403.3 ± 377 | 1441 ± 317 |
| Platelet count (× 109/L) | 1710.7 ± 66 | 1577.3 ± 94 | 1580.0 ± 17 | 1480.0 ± 17 |
| MPV # (fL) | 10.5 ± 0.9 | 10.3 ± 0.9 | 10.3 ± 0.95 | 9.8 ± 0.65 |
| WBC # (× 106/L) | 2.1 ± 1.5 | n.d. | 1.7 ± 1.5 | n.d. |
| RBC # (× 109/L) | 1.4 ± 0.26 | n.d. | 1.4 ± 0.75 | n.d. |
| CD42b (MFI) | 63.3 ± 12 | 51.9 ± 20 | 61.6 ± 20 | 57.8 ± 10 |
| CD61 (MFI) | 58.3 ± 14 | 48.7 ± 18 | 63.1 ± 18 | 53.8 ± 18 |
| P-Selectin (%) | 5.7 ± 1.1 | 5.0 ± 1.7 | 7.6± 1.7 | 7.0 ± 2.8 |
| NEU1 (MFI) | 51.9 ± 6.4 | 50.8 ± 16 | 111.8 ± 27∗∗∗ | 111.0 ± 14.3∗∗∗ |
| Annexin V (%) | 2.2 ± 1.4 | 1.29 ± 2.1 | 5.6 ± 2.6∗ | 6.6 ± 2.2∗ |
| RCA I (MFI) | 401.4 ± 176 | 382.3 ± 166 | 714.1 ± 264∗∗ | 638.7 ± 269∗∗ |
| ECL (MFI) | 88.1 ± 21 | 88.4 ± 18 | 137.7 ± 43∗∗ | 131.1 ± 28∗∗ |
| Con A (MFI) | 47.9 ± 4.4 | 47.4 ± 4 | 149.6 ± 27∗∗∗ | 150.7 ± 25∗∗∗ |
| pH | 7.4 ± 0.03 | n.d. | 7.3 ± 0.05 | n.d. |
| . | Day 0 (PC) . | Day 0 (isolated) . | Day 7 (PC) . | Day 7 (isolated) . |
|---|---|---|---|---|
| Platelet count # (× 109/L) | 1507.6 ± 373 | 1516 ± 284 | 1403.3 ± 377 | 1441 ± 317 |
| Platelet count (× 109/L) | 1710.7 ± 66 | 1577.3 ± 94 | 1580.0 ± 17 | 1480.0 ± 17 |
| MPV # (fL) | 10.5 ± 0.9 | 10.3 ± 0.9 | 10.3 ± 0.95 | 9.8 ± 0.65 |
| WBC # (× 106/L) | 2.1 ± 1.5 | n.d. | 1.7 ± 1.5 | n.d. |
| RBC # (× 109/L) | 1.4 ± 0.26 | n.d. | 1.4 ± 0.75 | n.d. |
| CD42b (MFI) | 63.3 ± 12 | 51.9 ± 20 | 61.6 ± 20 | 57.8 ± 10 |
| CD61 (MFI) | 58.3 ± 14 | 48.7 ± 18 | 63.1 ± 18 | 53.8 ± 18 |
| P-Selectin (%) | 5.7 ± 1.1 | 5.0 ± 1.7 | 7.6± 1.7 | 7.0 ± 2.8 |
| NEU1 (MFI) | 51.9 ± 6.4 | 50.8 ± 16 | 111.8 ± 27∗∗∗ | 111.0 ± 14.3∗∗∗ |
| Annexin V (%) | 2.2 ± 1.4 | 1.29 ± 2.1 | 5.6 ± 2.6∗ | 6.6 ± 2.2∗ |
| RCA I (MFI) | 401.4 ± 176 | 382.3 ± 166 | 714.1 ± 264∗∗ | 638.7 ± 269∗∗ |
| ECL (MFI) | 88.1 ± 21 | 88.4 ± 18 | 137.7 ± 43∗∗ | 131.1 ± 28∗∗ |
| Con A (MFI) | 47.9 ± 4.4 | 47.4 ± 4 | 149.6 ± 27∗∗∗ | 150.7 ± 25∗∗∗ |
| pH | 7.4 ± 0.03 | n.d. | 7.3 ± 0.05 | n.d. |
Platelet count indicated by #, WBC and RBC count were measured using an automated hemoanalyzer (Sysmex). Platelet count was also measured using flow cytometry using reference beads. Platelet surface receptors CD42b (GPIbα) and CD61, the α-granule marker P-selectin, and NEU1 were measured by flow cytometry using specific mAB. PS exposure was measured by flow cytometry using annexin V. Surface terminal galactose was measured using RCA I and ECL lectins. % indicated positive events compared with control. Data are presented as mean ± standard deviation from 7 donor platelet concentrates. The degree of significance is indicated ∗P < .05, ∗∗ P < .01, ∗∗∗P < .001.
MPV, mean platelet volume; n.d., no data; PC, platelet concentrates; RBC, red blood cell; WBC, white blood cell.