Typical antibody detection (screening) results for autoantibodies and alloantibodies
| Reagent red cells . | Cold autoantibody . | Warm autoantibody . | No antibody detected . | Alloantibody detected . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| RT . | 37C . | IAT . | RT . | 37C . | IAT . | RT . | 37C . | IAT . | RT . | 37C . | IAT . | |
| 1 | 4+ | 3+ | 1+ | 0 | 0 | 3+ | 0 | 0 | 0 | 0 | 0 | 0 |
| 2 | 4+ | 3+ | 1+ | 0 | 0 | 3+ | 0 | 0 | 0 | 0 | 0 | 3+ |
| 3 | 4+ | 3+ | 1+ | 0 | 0 | 3+ | 0 | 0 | 0 | 0 | 0 | 0 |
| Autocontrol* | 4+ | 3+ | 1+ | 0 | 0 | 4+ | 0 | 0 | 0 | 0 | 0 | 0 |
| Reagent red cells . | Cold autoantibody . | Warm autoantibody . | No antibody detected . | Alloantibody detected . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| RT . | 37C . | IAT . | RT . | 37C . | IAT . | RT . | 37C . | IAT . | RT . | 37C . | IAT . | |
| 1 | 4+ | 3+ | 1+ | 0 | 0 | 3+ | 0 | 0 | 0 | 0 | 0 | 0 |
| 2 | 4+ | 3+ | 1+ | 0 | 0 | 3+ | 0 | 0 | 0 | 0 | 0 | 3+ |
| 3 | 4+ | 3+ | 1+ | 0 | 0 | 3+ | 0 | 0 | 0 | 0 | 0 | 0 |
| Autocontrol* | 4+ | 3+ | 1+ | 0 | 0 | 4+ | 0 | 0 | 0 | 0 | 0 | 0 |
Results after mixing patient plasma with reagent RBCs. Agglutination is graded 1-4+; no agglutination is indicated as 0.
Autocontrol contains patient plasma plus patient RBCs.
Italics indicate typical optimum reactivity of cold autoantibody. Bold indicates typical optimum reactivity of warm autoantibody. Italics and bold indicate typical reactivity of alloantibody.
RT, room temperature (direct agglutination/immediate spin at room temperature); 37C, results after incubation at 37 °C for 10-30 minutes; IAT, results after addition of AHG.