Percentages of CD45.2 donor cells in CD45.1-recipient mice after transplantation of murine HSC cells either treated with rhFTVI or with buffer alone
| Experiment, n . | Cell*/treatment . | Treatment . | Mice, n . | Days Post Transplantation, % . | ||
|---|---|---|---|---|---|---|
| 10 d . | 18 d . | 30 d . | ||||
| 1 | Flk2−CD34+ | HBSS | 2 | 5.3, 30 | 24, 50 | 18, 8 |
| Flk2−CD34+ | rhFTVI | 3 | 40, 15, 20 | 15.8, 20, 18.7 | 36, 37, 41 | |
| Flk2−CD34− | HBSS | 3 | 15, 19, 20 | 37, 55, 56 | 73, 88, 59 | |
| Flk2−CD34− | rhFTVI | 2 | 2, 20 | 10, 80 | 50, 68 | |
| 2 | Flk2−CD34+ | HBSS | 3 | 2.7, 16, 3.7 | 5, 10.2, 70 | 34, 33.6, 31 |
| Flk2−CD34+ | rhFTVI | 5 | 1, 2.9, 1, 1.3, 1.2 | 4, 10, 17, 3, 19.4 | 29, 31, 29, 23, 44.5 | |
| Flk2−CD34− | HBSS | 5 | 2.2, 4.3, 3, 1, 2 | 23, 10.3, 17, 70, 15 | 67, 39, 67, 69, 50 | |
| Flk2−CD34− | rhFTVI | 5 | 0.3, 3.3, 1.5 | 15, 8, 71 | 33, 27, 50 | |
| 3 | Flk2−CD34+ | HBSS | 9 | 6.3, 14.1, 55, 55, 33.5, 18, 21.6, 15.4, 14 | 11, 28.8, 12.8, 5.3, 6.4, 15, 17.1, 11.6, 4.1 | 3.2, 1, 2.4, 20, 0.9, 3.4, 1.8, 4, 3.8 |
| Flk2−CD34+ | rhFTVI | 8 | 10.1, 9.8, 4.5, 30, 15.3, 22, 4, 20 | 8.3, 35.5, 1, 27, 26.7, 9, 60.4, 20. 35.5 | 42, 10, 25, 1.5, 21.5, 2.2, 15, 16.1 | |
| Flk2−CD34− | HBSS | 8 | 35, 6.1, 10, 3.2, 13.1, 8, 44.6, 16.8 | 35, 67, 60.4, 69, 65, 9, 40, 58 | 65, 86.9, 70.9, 74,5, 75.7, 45.2, 70.4, 68.9 | |
| Flk2−CD34− | rhFTVI | 10 | 51, 1.5, 9.6, 50.9, 65.4, 6.3, 14.1, 55, 33.5, 31.5 | 59, 70, 62.7, 85.2, 67.4, 52, 50, 53.5, 80, 66 | 67.1, 63.1, 62.5, 63.1, 62.5, 74.4, 65.6, 71, 70.3, 62.5, 61.3, 70.2 | |
| Experiment, n . | Cell*/treatment . | Treatment . | Mice, n . | Days Post Transplantation, % . | ||
|---|---|---|---|---|---|---|
| 10 d . | 18 d . | 30 d . | ||||
| 1 | Flk2−CD34+ | HBSS | 2 | 5.3, 30 | 24, 50 | 18, 8 |
| Flk2−CD34+ | rhFTVI | 3 | 40, 15, 20 | 15.8, 20, 18.7 | 36, 37, 41 | |
| Flk2−CD34− | HBSS | 3 | 15, 19, 20 | 37, 55, 56 | 73, 88, 59 | |
| Flk2−CD34− | rhFTVI | 2 | 2, 20 | 10, 80 | 50, 68 | |
| 2 | Flk2−CD34+ | HBSS | 3 | 2.7, 16, 3.7 | 5, 10.2, 70 | 34, 33.6, 31 |
| Flk2−CD34+ | rhFTVI | 5 | 1, 2.9, 1, 1.3, 1.2 | 4, 10, 17, 3, 19.4 | 29, 31, 29, 23, 44.5 | |
| Flk2−CD34− | HBSS | 5 | 2.2, 4.3, 3, 1, 2 | 23, 10.3, 17, 70, 15 | 67, 39, 67, 69, 50 | |
| Flk2−CD34− | rhFTVI | 5 | 0.3, 3.3, 1.5 | 15, 8, 71 | 33, 27, 50 | |
| 3 | Flk2−CD34+ | HBSS | 9 | 6.3, 14.1, 55, 55, 33.5, 18, 21.6, 15.4, 14 | 11, 28.8, 12.8, 5.3, 6.4, 15, 17.1, 11.6, 4.1 | 3.2, 1, 2.4, 20, 0.9, 3.4, 1.8, 4, 3.8 |
| Flk2−CD34+ | rhFTVI | 8 | 10.1, 9.8, 4.5, 30, 15.3, 22, 4, 20 | 8.3, 35.5, 1, 27, 26.7, 9, 60.4, 20. 35.5 | 42, 10, 25, 1.5, 21.5, 2.2, 15, 16.1 | |
| Flk2−CD34− | HBSS | 8 | 35, 6.1, 10, 3.2, 13.1, 8, 44.6, 16.8 | 35, 67, 60.4, 69, 65, 9, 40, 58 | 65, 86.9, 70.9, 74,5, 75.7, 45.2, 70.4, 68.9 | |
| Flk2−CD34− | rhFTVI | 10 | 51, 1.5, 9.6, 50.9, 65.4, 6.3, 14.1, 55, 33.5, 31.5 | 59, 70, 62.7, 85.2, 67.4, 52, 50, 53.5, 80, 66 | 67.1, 63.1, 62.5, 63.1, 62.5, 74.4, 65.6, 71, 70.3, 62.5, 61.3, 70.2 | |
cell number = ∼800 to 1000.