Table 2. Characterization of Xase enzyme... | American Society of Hematology

Table 2.

Characterization of Xase enzyme kinetics

	This study			Previously reported
	K_m,app (nM)	V_max (nM fXa/min)	k_cat (min⁻¹)	K_m,app (nM)	V_max (nM fXa/min)	k_cat (min⁻¹)
−ND	N.D.	0.014 ± 0.009	0.014 ± 0.009	—	—	—
+ND	139 ± 75	1.03 ± 0.32	1.03 ± 0.32	20-60^{52,77,116,117}	1-25^{52,77,116,117}	136-1740¹¹⁸

	This study			Previously reported
	K_m,app (nM)	V_max (nM fXa/min)	k_cat (min⁻¹)	K_m,app (nM)	V_max (nM fXa/min)	k_cat (min⁻¹)
−ND	N.D.	0.014 ± 0.009	0.014 ± 0.009	—	—	—
+ND	139 ± 75	1.03 ± 0.32	1.03 ± 0.32	20-60^{52,77,116,117}	1-25^{52,77,116,117}	136-1740¹¹⁸

Values were averaged from 2 or more independent experiments ±SE in the absence (-ND) and presence (+ND) of 10 µM nanodiscs.

K_m,app, apparent Michael–Menten constant; N.D., not detectable; V_max, maximum enzyme velocity.