Apparent binding kinetics and affinities of fVIIIa, fIXa, and Xase with lipid nanodiscs
| . | This study . | Previously reported . | ||
|---|---|---|---|---|
| kon (×104 M−1 s−1) . | koff (×10−3 s−1) . | KD (nM) . | KD (nM) . | |
| fVIIIa | 8.62 ± 0.11 | 2.79 ± 0.55 | 32.3 ± 6.0 | 2-55,76,113 |
| fIXa | 36.3 ± 1.2 | 72.3 ± 3.0 | 199 ± 1.7 | 12-100081,114,115 |
| Xase | 14.9 ± 3.5 | 10.7 ± 0.90 | 71.3 ± 4.4 | 0.577 |
| . | This study . | Previously reported . | ||
|---|---|---|---|---|
| kon (×104 M−1 s−1) . | koff (×10−3 s−1) . | KD (nM) . | KD (nM) . | |
| fVIIIa | 8.62 ± 0.11 | 2.79 ± 0.55 | 32.3 ± 6.0 | 2-55,76,113 |
| fIXa | 36.3 ± 1.2 | 72.3 ± 3.0 | 199 ± 1.7 | 12-100081,114,115 |
| Xase | 14.9 ± 3.5 | 10.7 ± 0.90 | 71.3 ± 4.4 | 0.577 |
All data represent the average of ≥3 independent experiments with a 95% confidence interval. kon, rate of association; koff, rate of dissociation; KD, dissociation constant (koff/kon).