EMSA results
| Probe . | Location (bp) . | Predicted motifs (5) . | Gel shift results . |
|---|---|---|---|
| 1/2 | −37/−19 | TATA box | No shifted species |
| 3/4 | −56/−39 | α-IRP −51/−42 site | Shifted species detected in EBV and K562 |
| 5/6 | −67/−50 | Sp1 −61/−56 site | No shifted species |
| 7/8 | −78/−61 | NFY −72/−65 site | Shifted species detected in EBV and K562; binding impaired by α-NFY antibody |
| 9/10 | −88/−72 | NF1 −85/−72 site | Shifted species detected in EBV and K562; binding impaired by α-NF1 antibody |
| 11/12 | −100/−83 | Three shifted species detected in EBV and K562; 1 shifted species only in K562 | |
| 13/14 | −128/−111 | Sp1 −121/−116 site | Three shifted species detected in EBV and K562; 1 shifted species only in K562 |
| Probe . | Location (bp) . | Predicted motifs (5) . | Gel shift results . |
|---|---|---|---|
| 1/2 | −37/−19 | TATA box | No shifted species |
| 3/4 | −56/−39 | α-IRP −51/−42 site | Shifted species detected in EBV and K562 |
| 5/6 | −67/−50 | Sp1 −61/−56 site | No shifted species |
| 7/8 | −78/−61 | NFY −72/−65 site | Shifted species detected in EBV and K562; binding impaired by α-NFY antibody |
| 9/10 | −88/−72 | NF1 −85/−72 site | Shifted species detected in EBV and K562; binding impaired by α-NF1 antibody |
| 11/12 | −100/−83 | Three shifted species detected in EBV and K562; 1 shifted species only in K562 | |
| 13/14 | −128/−111 | Sp1 −121/−116 site | Three shifted species detected in EBV and K562; 1 shifted species only in K562 |