| Optimal material | • Material from primary diagnosis (aspirate, biopsy) • Sufficient material and blast content • Selection of correct material (PB/BM) |
| Skilled laboratory | • Experience (number of ALL cases) • Participation in quality-control rounds • International standards for methods |
| Reporting of results | • Timely and reliable reporting • Results for each time point should include ○ Level of MRD ○ Sensitivity in case of a negative result ○ Further specification in case of nonquantifiable MRD |
| Terminology for MRD response | • MRD response is evaluated in patients with hematologic CR • Clear definition of results similar to definition of hematologic CR6,7 MRD CR: ○ MRD-negative with a minimum sensitivity of 0.01% MRD failure: ○ MRD-positive ≥0.01% MRD intermediate: ○ All other types of results, eg, ○ MRD-positive below 0.01% ○ Nonquantifiable but detectable MRD below 0.01% (PNQ) ○ MRD-negative with insufficient sensitivity |
| Optimal material | • Material from primary diagnosis (aspirate, biopsy) • Sufficient material and blast content • Selection of correct material (PB/BM) |
| Skilled laboratory | • Experience (number of ALL cases) • Participation in quality-control rounds • International standards for methods |
| Reporting of results | • Timely and reliable reporting • Results for each time point should include ○ Level of MRD ○ Sensitivity in case of a negative result ○ Further specification in case of nonquantifiable MRD |
| Terminology for MRD response | • MRD response is evaluated in patients with hematologic CR • Clear definition of results similar to definition of hematologic CR6,7 MRD CR: ○ MRD-negative with a minimum sensitivity of 0.01% MRD failure: ○ MRD-positive ≥0.01% MRD intermediate: ○ All other types of results, eg, ○ MRD-positive below 0.01% ○ Nonquantifiable but detectable MRD below 0.01% (PNQ) ○ MRD-negative with insufficient sensitivity |