Fluorescence intensities of T-cell subpopulations as in-sample quality control
| Antigen and label (population) . | MedFI . | Intracenter CV . | Intercenter CV . | P . |
|---|---|---|---|---|
| CD4 PacB (CD4+CD3+) | 6 707 | 22.6% | 27.2% | .9 |
| CD3 APC (CD4+CD3+) | 38 786 | 30.2% | 32.8% | 1.0 |
| CD45 PacO (CD4+CD3+) | 5 441 | 21.2% | 25.6% | .6 |
| CD5 PerCP Cy5.5 (CD4+CD3+) | 11 684 | 22.5% | 30.7% | .2 |
| CD8 FITC (CD8+CD3+) | 13 182 | 23.4% | 26.5% | .0 |
| Antigen and label (population) . | MedFI . | Intracenter CV . | Intercenter CV . | P . |
|---|---|---|---|---|
| CD4 PacB (CD4+CD3+) | 6 707 | 22.6% | 27.2% | .9 |
| CD3 APC (CD4+CD3+) | 38 786 | 30.2% | 32.8% | 1.0 |
| CD45 PacO (CD4+CD3+) | 5 441 | 21.2% | 25.6% | .6 |
| CD5 PerCP Cy5.5 (CD4+CD3+) | 11 684 | 22.5% | 30.7% | .2 |
| CD8 FITC (CD8+CD3+) | 13 182 | 23.4% | 26.5% | .0 |
MedFI of all CD4+CD3+ (n = 650) and CD8+CD3+ (n = 652) populations, mean intracenter CV, and intercenter CV. P values relate to the comparison between intra- and intercenter CV.
APC, allophycocyanin; CV, coefficient of variation; FITC, fluorescein isothiocyanate; PacB, Pacific Blue; PacO, Pacific Orange; PerCP, peridinin-chlorophyll-protein.