Primers and conditions for quantitative polymerase chain reaction
| Gene . | Sequence (5'-3') . | Annealing T (°C) . | Product (bp) . |
|---|---|---|---|
| GAPDH | F: GCACCGTCAAGGCTGAGAAC | 60 | 138 |
| R: TGGTGAAGACGCCAGTGGA | |||
| CXCR5 | F: ATCGCCGTGGACCGCTACC | 60 | 84 |
| R: ACAGGTGATGTGGATGGAGAGGAG | |||
| BAFF-R | F: CTGTCTCCGGGAATCTCTGATG | 60 | 70 |
| R: GGGTGGTTCCTGGGTCTTCC | |||
| BCMA | F: CATGCTTGCATACCTTGTCAACTTCG | 60 | 120 |
| R: GGTCCAGAGAATCGCATTCGTTCC | |||
| TACI | F: AGCAAGGCAAGTTCTATGACCATCTC | 60 | 157 |
| R: ACTTCTCCACTCCGCTGTCTCC | |||
| XBP1 | F: CCTGGTTGCTGAAGAGGAGG | 60 | 145 |
| R: CCATGGGGAGATGTTCTGGAG | |||
| Pax5 | F: GCCTAGCGTCAGTTCCATCAACAG | 60 | 123 |
| R: ACCGAGGACACCTGCGTCAC | |||
| IRF4 | F: GAGCAATGACTTTGAGGAACTG | 60 | 205 |
| R: CATCATGTAGTTGTGAACCTGC | |||
| PRDM1 | F: CCCTCTGAAGAAACAGAATG | 60 | 241 |
| R: GCTTGTGCTGCTAAATCTCT |
| Gene . | Sequence (5'-3') . | Annealing T (°C) . | Product (bp) . |
|---|---|---|---|
| GAPDH | F: GCACCGTCAAGGCTGAGAAC | 60 | 138 |
| R: TGGTGAAGACGCCAGTGGA | |||
| CXCR5 | F: ATCGCCGTGGACCGCTACC | 60 | 84 |
| R: ACAGGTGATGTGGATGGAGAGGAG | |||
| BAFF-R | F: CTGTCTCCGGGAATCTCTGATG | 60 | 70 |
| R: GGGTGGTTCCTGGGTCTTCC | |||
| BCMA | F: CATGCTTGCATACCTTGTCAACTTCG | 60 | 120 |
| R: GGTCCAGAGAATCGCATTCGTTCC | |||
| TACI | F: AGCAAGGCAAGTTCTATGACCATCTC | 60 | 157 |
| R: ACTTCTCCACTCCGCTGTCTCC | |||
| XBP1 | F: CCTGGTTGCTGAAGAGGAGG | 60 | 145 |
| R: CCATGGGGAGATGTTCTGGAG | |||
| Pax5 | F: GCCTAGCGTCAGTTCCATCAACAG | 60 | 123 |
| R: ACCGAGGACACCTGCGTCAC | |||
| IRF4 | F: GAGCAATGACTTTGAGGAACTG | 60 | 205 |
| R: CATCATGTAGTTGTGAACCTGC | |||
| PRDM1 | F: CCCTCTGAAGAAACAGAATG | 60 | 241 |
| R: GCTTGTGCTGCTAAATCTCT |